Imaging flow cytometry with a real-time throughput beyond 1,000,000 events per second

作     者：Jiehua Zhou Liye Mei Mingjie Yu Xiao Ma Dan Hou Zhuo Yin Xun Liu Yan Ding Kaining Yang Ruidong Xiao Xiandan Yuan Yueyun Weng Mengping Long Taobo Hu Jinxuan Hou Yu Xu Liang Tao Sisi Mei Hui Shen Yaxiaer Yalikun Fuling Zhou Liang Wang Du Wang Sheng Liu Cheng Lei Jiehua Zhou;Liye Mei;Mingjie Yu;Xiao Ma;Dan Hou;Zhuo Yin;Xun Liu;Yan Ding;Kaining Yang;Ruidong Xiao;Xiandan Yuan;Yueyun Weng;Mengping Long;Taobo Hu;Jinxuan Hou;Yu Xu;Liang Tao;Sisi Mei;Hui Shen;Yaxiaer Yalikun;Fuling Zhou;Liang Wang;Du Wang;Sheng Liu;Cheng Lei

作者机构：The Institute of Technological SciencesWuhan UniversityWuhan 430072China School of Computer ScienceHubei University of TechnologyWuhan 430068China Division of Materials ScienceNara Institute of Science and TechnologyTakayama-cho 8916-5Japan School of ScienceHubei University of TechnologyWuhan 430068China Department of PathologyPeking University Cancer HospitalBeijing 100142China Department of Breast SurgeryPeking University People’s HospitalBeijing 100044China Department of Thyroid and Breast SurgeryZhongnan HospitalWuhan UniversityWuhan 430071China Department of Radiation and Medical OncologyZhongnan HospitalWuhan UniversityWuhan 430071China People’s Hospital of Anshun City Guizhou ProvinceAnshun 561000China Department of HematologyZhongnan HospitalWuhan UniversityWuhan 430071China National Engineering Laboratory for Next Generation Internet Access SystemSchool of Optics and Electronic InformationHuazhong University of Science and TechnologyWuhan 430074China Suzhou Institute of Wuhan UniversitySuzhou 215000China Shenzhen Institute of Wuhan UniversityShenzhen 518057China 

出 版 物：《Light(Science & Applications)》 (光(科学与应用)(英文版))

年 卷 期：2025年第14卷第4期

页      面：931-947页

核心收录：

学科分类：07[理学] 0702[理学-物理学] 

基　　金：supported by the National Key R&D Program of China(2023YFF0723300) National Natural Science Foundation of China(62475198,62075200,12374295) Fundamental Research Funds for the Central Universities(2042024kf0003,2042024kf1010,2042023kf0105) Hubei Provincial Natural Science Foundation of China(2023AFB133) Jiangsu Science and Technology Program(BK20221257) Shenzhen Science and Technology Program(JCYJ20220530140601003,JCYJ20230807090207014) Translational Medicine and Multidisciplinary Research Project of Zhongnan Hospital of Wuhan University(ZNJC202217,ZNJC202232) The Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCYR-2022-006) Hubei Province Young Science and Technology Talent Morning Hight Lift Project(202319) The Fund of National Key Laboratory of Plasma Physics(6142A04230201) We gratefully acknowledge Serendipity Lab for facilitating collaboration opportunities 

主　　题：real time data processing flow cytometryoffering spatial resolution imaging flow cytometry ifc combines high throughput cell analysis cell analysis imaging flow cytometry optical time stretch imaging 

摘      要：Imaging flow cytometry(IFC)combines the imaging capabilities of microscopy with the high throughput of flow cytometry,offering a promising solution for high-precision and high-throughput cell analysis in fields such as biomedicine,green energy,and environmental ***,due to limitations in imaging framerate and realtime data processing,the real-time throughput of existing IFC systems has been restricted to approximately 1000-10,000 events per second(eps),which is insufficient for large-scale cell *** this work,we demonstrate IFC with real-time throughput exceeding 1,000,000 eps by integrating optical time-stretch(OTS)imaging,microfluidic-based cell manipulation,and online image *** flowing at speeds up to 15 m/s are clearly imaged with a spatial resolution of 780 nm,and images of each individual cell are captured,stored,and *** capabilities and performance of our system are validated through the identification of malignancies in clinical colorectal *** work sets a new record for throughput in imaging flow cytometry,and we believe it has the potential to revolutionize cell analysis by enabling highly efficient,accurate,and intelligent measurement.