LG1 promotes preligule band formation through directly activating ZmPIN1 genes in maize

作     者：Zhuojun Zhong Minhao Yao Yingying Cao Dexin Kong Baobao Wang Yanli Wang Rongxin Shen Haiyang Wang Qing Liu Zhuojun Zhong;Minhao Yao;Yingying Cao;Dexin Kong;Baobao Wang;Yanli Wang;Rongxin Shen;Haiyang Wang;Qing Liu

作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesGuangdong Laboratory for Lingnan Modern AgricultureCollege of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijing 100081China Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative UtilizationCollege of Horticultural Science&TechnologyHeBei Normal University of Science&TechnologyQinhuangdaoHebei 066004China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报(英文版))

年 卷 期：2025年第52卷第3期

页      面：356-366页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Key Research and Development Program of China(2021YFF1000301) the National Natural Science Foundation of China(32472179,32130077,32201835) the Natural Science Foundation of Hebei Province(C2022407068) the Natural Science Foundation of Guangdong Province(2024A1515030237,2022A1515011002) the Hainan Yazhou Bay Seed Lab(B21HJ8101) 

主　　题：Maize(Zea mays L.) LG1 Leaf angle(LA) Preligule band Auxin transport 

摘      要：Increasing plant density is an effective strategy for enhancing crop yield per unit land area.A key architectural trait for crops adapting to high planting density is a smaller leaf angle(LA).Previous studies have demonstrated that LG1,a SQUAMOSA BINDING PROTEIN(SBP)transcription factor,plays a critical role in LA ***,the molecular mechanisms underlying the regulation of LG1 on LA formation remain largely *** this study,we conduct comparative RNA-seq analysis of the preligule band(PLB)region of wild type and lg1 mutant *** Ontology(GO)term enrichment analysis reveals enrichment of phytohormone pathways and transcription factors,including three auxin transporter genes ZmPIN1a,ZmPIN1b,and *** molecular experiments demonstrate that LG1 can directly bind to the promoter region of these auxin transporter genes and activate their *** also show that double and triple mutants of these ZmPINs genes exhibit varying degrees of auricle size reduction and thus decreased *** the contrary,overexpression of ZmPIN1a causes larger auricle and *** together,our findings establish a functional link between LG1 and auxin transport in regulating PLB formation and provide valuable targets for genetic improvement of LA for breeding high-density tolerant maize cultivars.