Inhibitory effects of cytosine deaminase gene-transfected bone marrow mesenchymal stem cells on glioma cell proliferation

作     者：Fei Song Qi Xing Kedong Song Jian Liu Guangchun Ji Yufang Ma Tianqing Liu Minghai Wei Xuehu Ma 

作者机构：Dalian R&D Center for Stem Cells and Tissue Engineering Dalian University of Technology Dalian 116024 Liaoning Province China Department of Neurosurgery Second Hospital Affiliated to Dalian Medical University Dalian 116027 Liaoning Province China Department of Anesthesiology First Hospital Affiliated to Dalian Medical University Dalian 116011 Liaoning Province China Department of Biochemistry and Molecular Biology Dalian Medical University Dalian 116044 Liaoning Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第16期

页      面：1238-1242页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：the Natural Science Foundation of Liaoning Province, No. 20092165 a grant from Education Department of Liaoning Province, No. 2008Z081 the Science and Technology Development Program of Dalian, No. 2008E13SF203 

主　　题：magnetic resonance imaging glioma gene therapy cytosine deaminase gene bone marrow mesenchymal stem cells lentivirus 

摘      要：Bone marrow mesenchymal stem cells were isolated from C57BL mice, transfected with the cytosine deaminase （CD） gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes. C57MSC-CD/eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells. C57MSC-CD/eGFP cells were then implanted into rat models of brain C6 glioma. Rats were also intraperitoneally injected with 5-fluorocytosine after 7 days. MSC-CD/eGFP cells were irregularly distributed at the margin of the glioma, as well as encased and reduced the volume of the glioma. CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.