Ultra-sensitive and specific detection of ascorbic acid using a laser-engraved graphene electrode modified with a molecularly imprinted polymer

作     者：Jin, Wei Yao, Handong Wang, Xuefeng Li, Ming Gao, Xinghua Chen, Wei Zhang, Yuan 

作者机构：Shanghai Univ Mat Genome Inst Shanghai Engn Res Ctr Integrated Circuits & Adv Di Shanghai 200444 Peoples R China Chinese Acad Sci State Key Lab Transducer Technol Shanghai Inst Microsyst & Informat Tehncol Shanghai 200050 Peoples R China Tongji Univ Sch Med Tongji Hosp Dept Emergency Shanghai 200065 Peoples R China 

出 版 物：《JOURNAL OF MATERIALS CHEMISTRY C》 (J. Mater. Chem. C)

年 卷 期：2025年第13卷第12期

页      面：6390-6398页

核心收录：

学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0702[理学-物理学] 

基　　金：National Key Research and Development Program of China [2021Yfb3200804] National Key Research and Development Program of China Key Program of the National Natural Science Foundation of China 

主　　题：Biomarkers 

摘      要：Accurate and sensitive detection of small molecule biomarkers, such as ascorbic acid (AA), is pivotal for clinical diagnosis of various diseases. Herein, we report the development of an integrated electrochemical sensor that specifically targets and analyzes the ultra-low concentration of AA. The sensor incorporates a combination of techniques, featuring a CO2 laser-engraved graphene (LEG) electrode that has been further enhanced through the application of molecularly imprinted poly-o-phenylenediamine (PPD). The laser-engraved approach yields a three-dimensional (3D) porous graphene network with large surface area and good conductivity, enabling sensitive detection of AA. Furthermore, the molecularly imprinted PPD layer provides a tailored recognition site for AA, enhancing the sensor s specificity towards the target molecule. The integrated PPD/LEG electrode demonstrates the capability to detect AA in the concentration range of 1 to 100 mu M and 10 to 100 nM, with a limit of detection (LOD) of 1.45 nM and a limit of quantification (LOQ) of 20.44 nM. The real urine sample detection indicates that the developed PPD/LEG electrode can effectively differentiate cases in the melanoma mouse model from the healthy control group. The combination of the LEG electrode with the molecularly imprinted polymer (MIP) technique presents a novel platform for integrated sensing systems, offering unprecedented accuracy and sensitivity in the detection of small molecule biomarkers at trace concentrations. This innovation offers the potential for early clinical detection of diseases, where timely and precise measurements of biomarkers are crucial for diagnosis and treatment.