Nanohydroxyapatite and liposomes-coated integral bilayer scaffold for osteochondral repair via mimicking the dual differentiation microenvironment of BMSCs

作     者：Zhao, Weikang Sun, Biemin Song, Yu Cao, Yuan Liu, Yichen Zhou, Dandan Zhou, Qiang Xie, Feng Huang, Wei Li, Xiaoxiao Li, Yuling Xu, Yanqin Wang, Yiyang 

作者机构：Department of Orthopedics The First Affiliated Hospital of Chongqing Medical University Chongqing404000 China Department of Orthopedics The Third Affiliated Hospital of Chongqing Medical University Chongqing401120 China Tissue Repairing and Biotechnology Research Center The Third Affiliated Hospital of Chongqing Medical University Chongqing401120 China College of Chemistry and Chemical Engineering Chongqing University Chongqing400044 China Department of Geriatric Medicine Jiulongpo People's Hospital of Chongqing Chongqing400050 China Laboratory of Biological Tissue Engineering and Digital Medicine Affiliated Hospital of North Sichuan Medical College Nanchong637000 China 

出 版 物：《Nano Materials Science》 (Nano. Mater. Sci.)

年 卷 期：2025年

核心收录：

基　　金：This work was supported by grants from the China Postdoctoral Science Foundation (2022TQ0397, 2022MD723744, 2022M710564, 2022M720603) Natural Science Foundation of China (No. 82272553, 82102571, 81974346, 82102578) Chongqing Municipal Medical Youth Talent Support Program, Chongqing, China (YXQN202408) Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-MSX0089, CSTB2022NSCQ-MSX0104, CSTB2024NSCQ-MSX0532) Joint Medical Research Project of Health Commission & Science and Technology Bureau of Chongqing, China (2024QNXM032) Special Project for the Central Government to Guide the Development of Local Science and Technology in Sichuan Province (2023ZYD0071) National Natural Science Foundation of Sichuan (24NSFSC1274). Project of Innovative Science Research for Postgraduate of Chongqing Municipal Education Committee, Chongqing, China (CYS22389, CYB240224).This work was supported by grants from the China Postdoctoral Science Foundation (2022TQ0397, 2022MD723744, 2022M710564, 2022M720603) Natural Science Foundation of China (No. 82272553, 82102571, 81974346, 8210257, 82472404) Chongqing Municipal Medical Youth Talent Support Program, Chongqing, China (YXQN202408) Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-MSX0089, CSTB2022NSCQ-MSX0104, CSTB2024NSCQ-MSX0532) Joint Medical Research Project of Health Commission & Science and Technology Bureau of Chongqing, China (2024QNXM032) Special Project for the Central Government to Guide the Development of Local Science and Technology in Sichuan Province (2023ZYD0071) National Natural Science Foundation of Sichuan (24NSFSC1274) Project of Innovative Science Research for Postgraduate of Chongqing Municipal Education Committee, Chongqing, China (CYS22389, CYB240224) National Natural Science Foundation of Sichuan (2024NSFSC0678) Research Project of the Affiliated Hospital of North Sichuan Medical College (2023ZD002, 2023-2ZD001, 2024JB001) Disciplines Construction Program of The Third Affiliated Hospital of Chongqing Medical University (KY23035, KY23041).IACUC Approval Certification, Approval number:IACUC-CQMU-2023-0128, Project title:Research on the formation mechanism of porous microstructure and the improvement of osteogenic ability in the degradation process of bone repair material, Funding source:National Science Foundation of China (NO.82102571). Principal investigator: Weikang Zhao Department: The First Affiliated Hospital of Chongqing Medical University, Application date:2023-05-15, Approval date:2023-05-23 

主　　题：Stem cells 

摘      要：Osteochondral defects pose an enormous challenge, and no satisfactory therapy is available to date due to the hierarchy of the native tissue consisting of articular cartilage and subchondral bone. Constructing a scaffold with biological function and biomimetic structure is the key to achieving a high-quality repair effect. Herein, a natural polymer-based bilayer scaffold with a porous architecture similar to that of osteochondral tissue is designed, involving the transforming growth factor-beta3-liposome-loaded upper layer for superficial cartilage regeneration and the nanohydroxyapatite-coated lower layer for subchondral bone rehabilitation. This research is conducted to evaluate the effects of nanoparticle-modified bilayer scaffold to mimic the hierarchical pro-chondrogenic and pro-osteogenic microenvironment for the recruited endogenous bone marrow mesenchymal stem cells. The fabricated composites were evaluated for mechanical, physicochemical, biological properties, in vitro and in vivo tissue regeneration potential. Overall, the current bilayer scaffold could regenerate a cartilage-bone integrated tissue with a seamless interfacial integration and exhibited superior tissue repair outcomes compared to other single layer scaffolds based on morphological, radiological and histological evaluation, verifying that this novel graft could be an effective approach to tissue-engineered analogs of cartilage-subchondral bone and offer new therapeutic opportunities for osteochondral defect-associated diseases. © 2025 Chongqing University