Bubblegrams Reveal the Inner Body of Bacteriophage φKZ

作     者：Wu, Weimin Thomas, Julie A. Cheng, Naiqian Black, Lindsay W. Steven, Alasdair C. 

作者机构：Natl Inst Arthrit & Musculoskeletal & Skin Dis NI Struct Biol Lab NIH Bethesda MD 20892 USA Univ Maryland Sch Med Dept Biochem & Mol Biol Baltimore MD 21201 USA 

出 版 物：《SCIENCE》 (科学)

年 卷 期：2012年第335卷第6065期

页      面：182-182页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：NIAID NIH HHS [AI11676, R01 AI011676, R21 AI011676] Funding Source: Medline Intramural NIH HHS [Z01 AR027015, Z01 AR027015-13] Funding Source: Medline 

主　　题：Capsid/ultrastructure Cryoelectron Microscopy DNA, Viral Image Processing, Computer-Assisted Nucleocapsid/ultrastructure Pseudomonas Phages/chemistry Pseudomonas Phages/radiation effects Pseudomonas Phages/ultrastructure Viral Proteins/analysis 

摘      要：Dense packing of macromolecules in cellular compartments and higher-order assemblies makes it difficult to pick out even quite large components in electron micrographs, despite nominally high resolution. Immunogold labeling and histochemical procedures offer ways to map certain components but are limited in their applicability. Here, we present a differential mapping procedure, based on the physical principle of protein’s greater sensitivity to radiation damage compared with that of nucleic acid.