Interaction between <i>Aeromonas veronii</i> and epithelial cells of spotted sand bass (<i>Paralabrax maculatofasciatus</i>) in culture

作     者：Guzman-Murillo, MA Merino-Contreras, ML Ascencio, F 

作者机构：Biol Res Ctr Dept Marine Pathol La Paz 23000 Baja Calif Sur Mexico 

出 版 物：《JOURNAL OF APPLIED MICROBIOLOGY》 (应用微生物学杂志)

年 卷 期：2000年第88卷第5期

页      面：897-906页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学] 

主　　题：气单胞菌属/致病力 气单胞菌属/生理学 抗体/免疫学 细菌黏附/药物作用 鲈鱼 细胞 培养的 酶联免疫吸附测定 上皮细胞/微生物学 鱼疾病/微生物学 鱼类 糖轭合物/药理学 革兰氏阴性菌感染/兽医学 动力学 乳铁蛋白/药理学 外源凝集素类/免疫学 外源凝集素类/代谢 时间因素 动物 

摘      要：An in vitro fish model to study the interaction between Aeromonas veronii and skin, gill and intestinal epithelial cells was developed using primary cultures of mucosal cells (isolated from healthy organisms). Primary cultures were exposed to Aeromonas veronii strain A186 isolated from a patient with severe gastrointestinal disease. Microbial adherence was assessed by a spectrophotometric evaluation of an enzyme-linked, biotin-streptavidin Aer. veronii cell-adhesion assay to confluent monolayers of epithelial cells on 96-well tissue culture plates. The three primary-culture cells are susceptible to Aer. veronii attachment, with the greatest binding affinity found in gills, and to a lesser extent, in skin and intestine epithelial. cells. Aer. veronii adherence was dependent on bacterial load and incubation time. The effect of glycoconjugates on Aer. veronii adhesion was investigated by pre-incubating Aer. veronii cells with monosaccharides, sialic acid-rich glycoproteins and sulphated polysaccharides. In addition, the participation of a 48-kDa Aer. veronii lectin (MCBP - mucosal constituents binding protein), with affinity for mucosal constituents, was evaluated as a putative adhesion factor of Aer. veronii to the mucosal epithelial cells of spotted sand bass by pre-incubating bacterial cells with rabbit polyclonal antibodies to Aer. veronii MCBP. Our study shows that primary-culture fish mucosal cells provide a suitable model for the study of the interactions between Aer. veronii and epithelial cells of the fish mucosa, and to study putative virulence factors of fish pathogens.