Individual cell migration serves as the driving force for optic vesicle evagination

作     者：Rembold, Martina Loosli, Felix Adams, Richard J. Wittbrodt, Joachim 

作者机构：European Mol Biol Lab Dev Biol Unit D-69117 Heidelberg Germany Univ Cambridge Dept Physiol Dev & Neurosci Cambridge CB2 3DY England 

出 版 物：《SCIENCE》 (科学)

年 卷 期：2006年第313卷第5790期

页      面：1130-1134页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：Medical Research Council [G0400709] Funding Source: Medline MRC [G0400709] Funding Source: UKRI 

主　　题：Animals Animals, Genetically Modified Animals, Genetically Modified Cell Movement Cell Shape Cell Shape Central Nervous System/embryology Epithelial Cells/cytology Epithelial Cells/physiology Eye/embryology Fish Proteins/genetics Fish Proteins/physiology Gastrula/cytology Homeodomain Proteins/genetics Homeodomain Proteins/physiology Image Processing, Computer-Assisted Microscopy, Confocal Morphogenesis Mutation Oryzias/embryology Oryzias/genetics Prosencephalon/embryology Retina/cytology Retina/embryology Stem Cell Transplantation Stem Cells/physiology 

摘      要：The cellular mechanisms underlying organ formation are largely unknown. We visualized early vertebrate eye morphogenesis at single-cell resolution by in vivo imaging in medaka ( Oryzias latipes). Before optic vesicle evagination, retinal progenitor cells (RPCs) modulate their convergence in a fate-specific manner. Presumptive forebrain cells converge toward the midline, whereas medial RPCs remain stationary, predetermining the site of evagination. Subsequent optic vesicle evagination is driven by the active migration of individual RPCs. The analysis of mutants demonstrated that the retina-specific transcription factor rx3 determines the convergence and migration behaviors of RPCs. Hence, the migration of individual cells mediates essential steps of organ morphogenesis.