Identification of the agg1 mutation responsible for negative phototaxis in a “wild-type” strain of Chlamydomonas reinhardtii

作     者：Ide, Takahiro Mochiji, Shota Ueki, Noriko Yamaguchi, Katsushi Shigenobu, Shuji Hirono, Masafumi Wakabayashi, Ken-ichi 

作者机构：Laboratory for Chemistry and Life Science Institute of Innovative Research Tokyo Institute of Technology Yokohama 226-8503 Japan Laboratory for Organismal Patterning RIKEN Center for Developmental Biology Kobe 650-0047 Hyogo Japan Department of Biological Sciences Graduate School of Science University of Tokyo Tokyo 113-0033 Japan Department of Biological Sciences Graduate School of Science and Engineering Chuo University Tokyo 112-8551 Japan Functional Genomics Facility National Institute for Basic Biology Okazaki 444-8585 Aichi Japan Department of Basic Biology Faculty of Life Science SOKENDAI (The Graduate University for Advanced Studies) Okazaki 444-8585 Aichi Japan Department of Frontier Bioscience Hosei University Tokyo 184-8584 Japan 

出 版 物：《Biochemistry and Biophysics Reports》 (Biochem. Biophys. Rep.)

年 卷 期：2016年第7卷

页      面：379-385页

基　　金：Japan Society for the Promotion of Science, JSPS, (14-733, 15H01206, 15H01314, 16K14752, 2015298, 25291058, 26650093) Japan Society for the Promotion of Science, JSPS 

主　　题：Chlamydomonas Next-generation sequence Phototaxis 

摘      要：The unicellular green alga Chlamydomonas reinhardtii is a model organism for various studies in biology. CC-124 is a laboratory strain widely used as a wild type. However, this strain is known to carry agg1 mutation, which causes cells to swim away from the light source (negative phototaxis), in contrast to the cells of other wild-type strains, which swim toward the light source (positive phototaxis). Here we identified the causative gene of agg1 (AGG1) using AFLP-based gene mapping and whole genome next-generation sequencing. This gene encodes a 36-kDa protein containing a Fibronectin type III domain and a CHORD-Sgt1 (CS) domain. The gene product is localized to the cell body and not to flagella or basal body. © 2016 The Authors