ShRNA-mediated silencing of the Ndc80 gene suppress cell proliferation and affected hepatitis B virus-related hepatocellular carcinoma

作     者：Liu, Bo Yao, Zhicheng Hu, Kunpeng Huang, He Xu, Shilei Wang, Qinliang Yang, Yang Ren, Jie 

作者机构：Sun Yat Sen Univ Affiliated Hosp 3 Dept Gen Surg Guangzhou 510530 Guangdong Peoples R China Sun Yat Sen Univ Affiliated Hosp 3 Dept Liver Transplantat Guangzhou 510530 Guangdong Peoples R China Sun Yat Sen Univ Affiliated Hosp 3 Dept Ultrasound Guangzhou 510530 Guangdong Peoples R China 

出 版 物：《CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY》 (临床胃肠病学与生物学)

年 卷 期：2016年第40卷第3期

页      面：297-303页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：National Natural Science Foundation of China Science and Technology Planning Project of Guangdong Province, China [2013B010404014] 

主　　题：Hepatitis B Virus-Related Hepatocellular Carcinoma Cell Proliferation Hepatitis B virus Ndc80 complex Chromosome Segregation Human-centered computing 

摘      要：Background: Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies in the world, and hepatitis B virus (HBV) has been well established to cause HCC. Ndc80 complex is a conserved mitotic regulator dedicated to ensuring faithful chromosome segregation and plays an important role in inducing tumor formation. However, its role in HCC caused by HBV infection remains unclear. Methods: Immunohistochemistry (IHC), Western blot (WB), and real-time qRT-PCR were used to measure the expression of Ndc80 in HBV-related HCC tissues. Ndc80-specific short hairpin RNA (shRNA) was used to knock-down Ndc80 expression in the hepatoma cell line HeG2 and HepG2.2.15, which is stable transcribed with HBV genome. Furthermore, the effect of Ndc80 on cellular proliferation and growth were examined, respectively. Results: The expression level of Ndc80 was remarkably up-regulated in HBV-related HCC tissues. Down-regulation of Ndc80 expression suppressed HBV replication. With cell counting and the MTS assay, cellular proliferation and growth of Ndc80 knocking-down cell line was shown to be effectively restrained. Conclusion: This study suggests that Ndc80 may play an important role in the process of HBV-related HCC, and that it may be a potential biological treatment target in the future. (C) 2015 Elsevier Masson SAS. All rights reserved.