In vitro collection and posttransfusion engraftment characteristics of MNCs obtained by using a new separator for autologous PBPC transplantation

作     者：Snyder, EL Baril, L Cooper, DL Min, K Mechanic, S Stoddart, L Burtness, B Seagraves, P Debelak, J Gudino, M McCullough, J 

作者机构：Yale Univ Sch Med Dept Lab Med New Haven CT 06510 USA Yale Univ Sch Med Dept Med New Haven CT 06510 USA Univ Minnesota Dept Pathol Minneapolis MN 55455 USA Baxter Healthcare Corp Round Lake IL 60073 USA 

出 版 物：《TRANSFUSION》 (输血)

年 卷 期：2000年第40卷第8期

页      面：961-967页

核心收录：

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：NHLBI NIH HHS [S-RO1-HL61223] Funding Source: Medline 

主　　题：抗原 CD34/血液 血样采集 细胞分离/仪器和设备 细胞分离/方法 造血干细胞移植 单核细胞/细胞学 单核细胞/免疫学 软件 时间因素 青少年 成年人 女(雌)性 人类 男(雄)性 中年人 

摘      要：BACKGROUND: A clinical study was performed to evaluate the peripheral blood progenitor cell (PBPC) collection, transfusion, and engraftment characteristics associated with use of a blood cell separator (Amicus, Baxter Healthcare). STUDY DESIGN AND METHODS: Oncology patients (n = 31) scheduled for an autologous PBPC transplant following myeloablative therapy were studied. PBPCs were mobilized by a variety of chemotherapeutic regimens and the use of G-CSF. As no prior studies evaluated whether PBPCs collected on the Amicus separator would be viable after transfusion, to ensure patient safety, PBPCs were first collected on another cell separator (CS-3000 Plus, Baxter) and stored as backup. The day after the CS-3000 Plus collections were completed, PBPC collections intended for transfusion were performed using the Amicus instrument. For each transplant, 2.5 x 10(6) CD34+ PBPCs per kg of body weight were transfused. RESULTS: Clinical data collected on the donors immediately before and after PBPC collection with the Amicus device were comparable to donor data similarly obtained for the CS-3000 Plus collections. While the number of CD34+ cells and the RBC volume in the collected products were equivalent for the two devices, the platelet content of the Amicus collections was significantly lower than that of the CS-3000 Plus collections (4.35 x 10(10) platelets/ bag vs. 6.61 x 10(10) platelets/bag, p500 PMNs per mu L and 9.7 +/- 1.5 days to attain a platelet count of 20,000 per mu L-equivalent to data in the literature. No CS-3000 Plus backup cells were transfused and no serious adverse events attributable to the Amicus device were encountered. CONCLUSIONS: The mean Amicus CD34+ cell collection efficiency was better (p0.05) th