Intracellular calcium dynamics during photolysis

作     者：Busch, NA Reiken, SR Toner, M Yarmush, ML 

作者机构：Massachusetts Gen Hosp Ctr Engn Med Lab Surg Sci & Engn Boston MA 02114 USA Shriners Burns Hosp Boston MA 02114 USA Harvard Univ Sch Med Boston MA 02114 USA 

出 版 物：《JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME》 (美国机械工程师学会汇刊：生物机械工程学杂志)

年 卷 期：1998年第120卷第5期

页      面：570-578页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：NIGMS NIH HHS [GM-07035] Funding Source: Medline 

主　　题：苯胺化合物/药代动力学 钙/代谢 钙通道阻滞药/药理学 钙通道/药物作用 钙通道/辐射效应 钙信号/药物作用 钙信号/辐射效应 黑色素瘤 金属卟啉类/药理学 镍/药理学 硝苯地平/药理学 光化学疗法 光解 光敏感药/药理学 光谱法 质量 基质辅助激光解吸电离 时间因素 肿瘤细胞 培养的/辐射效应 维拉帕米/药理学 呫吨类/药代动力学 人类 

摘      要：The objective of this investigation was to gain a deeper understanding of the intracellular events that precede photolysis of cells. A model system, consisting of malignant melanoma cells pretreated,vith the calcium sensitive fluorescent dye, Fluo-3, was used to examine the intracellular calcium dynamics in single-cell photolysis experiments. Exposure of the cells to 632 nm laser light in the presence of photosensitizer, tin chlorin e6, resulted in a rise in intracellular calcium. The increase in intracellular calcium,was blocked using a variety of calcium channel blocking agents, including verapamil, nifedipine, and nickel. Treatment with the channel blockers was also effective in either decreasing or eliminating cell death despite the presence of lethal doses of photosensitizer and irradiation. These results show that intracellular calcium rises prior to plasma membrane lysis, and that this early rise in intracellular calcium is necessary for membrane rupture.