Structured light sheet fluorescence microscopy based on four beam interference

作     者：Lei, Ming Zumbusch, Andreas 

作者机构：Univ Konstanz Dept Chem D-78457 Constance Germany Chinese Acad Sci State Key Lab Transient Opt & Photon Xian Inst Opt & Precis Mech Xian 710119 Peoples R China 

出 版 物：《OPTICS EXPRESS》 (Opt. Express)

年 卷 期：2010年第18卷第18期

页      面：19232-19241页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：Center for Applied Photonics (CAP) at the University of Konstanz German Federal Ministry of Education and Research Chinese Academy of Sciences (CAS)/State Administration of Foreign Experts Affairs of China (SAFEA) 

主　　题：Confocal microscopy Fluorescence microscopy Image processing algorithms Light sheet microscopy Multiphoton microscopy Structured illumination microscopy 

摘      要：A 3D structured light sheet microscope using a four-faceted symmetric pyramid is presented. The sample is illuminated by the resulting four beam interference field. This approach combines advantages of standing wave and structured illumination microscopy. Examples of micrographs of fluorescently labeled Chinese hamster ovary (CHO) cells as well as of the compound eyes of drosophila are shown and the optical sectioning ability of our system is demonstrated. The capabilities and the limitations of the scheme are discussed. (C) 2010 Optical Society of America