Proteome Analysis of the Two-Component SalK/SalR System in Epidemic <i>Streptococcus suis</i> Serotype 2

作     者：Shen, Xiaodong Zhong, Qiu Zhao, Yan Yin, Supeng Chen, Tian Hu, Fuquan Li, Ming 

作者机构：Third Mil Med Univ Dept Microbiol Chongqing 400038 Peoples R China Third Mil Med Univ Dept Biochem & Mol Biol Chongqing 400038 Peoples R China 

出 版 物：《CURRENT MICROBIOLOGY》 (当今微生物学)

年 卷 期：2013年第67卷第1期

页      面：118-122页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：National Natural Science Foundation of China [30901282  81201330] 

主　　题：STREPTOCOCCUS suis SEROTYPES EPIDEMICS GENOMES PHENOTYPE MICROARRAY technology GENETIC transcription 

摘      要：Streptococcus suis serotype 2 (SS2) is a major swine pathogen of significant commercial importance worldwide and an emerging zoonotic agent of human streptococcal toxic shock-like syndrome. We previously reported that the Chinese highly pathogenic SS2 strains specifically harbor an 89K pathogenicity island, and the SalK/SalR two-component system encoded within this island mediates a piglet-lethal phenotype. However, its regulatory mechanism remains obscure. In the present study, we performed a proteome analysis to identify the target proteins regulated by the SalK/SalR system. This expression profiling analysis reveals 14 down-regulated proteins and 1 up-regulated protein. In partial accord with our previous microarray analysis, 9 out of the 14 down-regulated genes are also repressed at the transcriptional level. From the 9 matched genes, 5 were selected and further subjected to gene knockout and experimental infection of mice. The results show that the selected 5 genes are potential virulence factors and participate to various extents in the pathogenesis of SS2 infection, and may contribute to the attenuation of virulence mediated by the SalK/SalR system.