Comparison of higher-order multiphoton signal generation and collection at the 1700-nm window based on transmittance measurement of objective lenses

作     者：Wen, Wenhui Wang, Yuxin Liu, Hongji Wang, Kai Qiu, Ping Wang, Ke 

作者机构：Shenzhen Univ Coll Optoelect Engn Key Lab Optoelect Devices & Syst Minist Educ & Guangdong Prov Shenzhen Peoples R China Shenzhen Univ Coll Phys & Energy Shenzhen Peoples R China 

出 版 物：《JOURNAL OF BIOPHOTONICS》 (生物光子学杂志)

年 卷 期：2018年第11卷第1期

页      面：2018 Jan;11(1)页

核心收录：

学科分类：0710[理学-生物学] 071011[理学-生物物理学] 07[理学] 0702[理学-物理学] 

基　　金：National Natural Science Foundation of China [61475103, 11404218] National Hi-Tech Research and Development Program of China [2015AA020515] Science and Technology Innovation Commission of Shenzhen [KQJSCX20160226194151, KQTD20150710165601017, JCYJ20160307150657874] Natural Science Foundation of SZU 

主　　题：5-photon fluorescence deep tissue fourth harmonic generation multiphoton microscopy 

摘      要：One benefit of excitation at the 1700-nm window is the more accessible modalities of multiphoton signal generation. It is demonstrated here that the transmittance performance of the objective lens is of vital importance for efficient higher-order multiphoton signal generation and collection excited at the 1700-nm window. Two commonly used objective lenses for multiphoton microscopy (MPM) are characterized and compared, one with regular coating and the other with customized coating for high transmittance at the 1700-nm window. Our results show that, fourth harmonic generation imaging of mouse tail tendon and 5-photon fluorescence of carbon quantum dots using the regular objective lens shows an order of magnitude signal higher than those using the customized objective lens. Besides, the regular objective lens also enables a 3-photon fluorescence imaging depth of 1600 mu m in mouse brain in vivo. Our results will provide guidelines for objective lens selection for MPM at the 1700-nm window.