Effects of allelochemical gramine on metabolic activity and ultrastructure of cyanobacterium microcystis aeruginosa

作     者：Hong, Y. Hu, H.Y. Sakoda, A. Sagehashi, M. 

作者机构：College of Environmental Science and Engineering Beijing Forestry University Beijing 100083 China Environmental Simulation and Pollution Control State Key Joint Laboratory Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China Institute of Industrial Science University of Tokyo Tokyo 1068558 Japan 

出 版 物：《World Academy of Science, Engineering and Technology》 (World Acad. Sci. Eng. Technol.)

年 卷 期：2010年第71卷

页      面：825-829页

核心收录：

主　　题：Enzymes 

摘      要：In this study, inhibition of Microcystis aeruginosa by antialgal alleochemical gramine, was studied by analyzing algal metabolic activity (represented by esterase and total dehydrogenase activities) and cell ultrastructure (showing morphological and ultrastructure alterations using transmission electron microscopy and DNA ladder analysis). After gramine exposure, esterase and total dehydrogenase activities were increased firstly but decreased later. In contrast with the controls, the cells exposed to gramine showed apparent ultrastructure alterations with thylakoids in breakage, phycobilins in decrease, lipid and cyanophycin granules abundant firstly but dissolved afterwards, DNA in fragementation. The occurrence of increase of metabolic activity and specific granules reflected that the resistance of cellular response to gramine was initiated. DNA fragementation associated with the increase of metabolic activity and specific granules hinted that gramine caused M. aeruginosa cells to initiate some morphotype of programmed cell death.