The effects of combined conventional treatment, oral antioxidants and essential fatty acids on sperm biology in subfertile men

作     者：Comhaire, FH Christophe, AB Zalata, AA Dhooge, WS Mahmoud, AMA Depuydt, CE 

作者机构：State Univ Ghent Hosp Dept Internal Med Endocrinol Sect B-9000 Ghent Belgium Mansoura Univ Fac Med Dept Biochem Mansoura Egypt Assiut Univ Hosp Dept Dermatol & Androl Assiut Egypt 

出 版 物：《PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS》 (前列腺素、白细胞三烯与必需脂肪酸)

年 卷 期：2000年第63卷第3期

页      面：159-165页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：Fund for Scientific Research Flanders Laxdale Ltd., (OK 2195/2) 

主　　题：乙酰半胱氨酸/投药和剂量 乙酰半胱氨酸/治疗应用 顶体反应 抗氧化剂/投药和剂量 抗氧化剂/治疗应用 细胞膜/代谢 DNA/代谢 脂肪酸类 必需/治疗应用 脂肪酸类 不饱和/代谢 自由基清除剂/投药和剂量 自由基清除剂/治疗应用 不育 男(雄)性/治疗 磷脂类/代谢 试点项目 妊娠率 前瞻性研究 活性氧 吸烟 精子计数 精子/药物作用 时间因素 维生素A/投药和剂量 维生素A/治疗应用 维生素E/投药和剂量 维生素E/治疗应用 成年人 女(雌)性 人类 男(雄)性 妊娠 

摘      要：We evaluated the effects of combined conventional treatment, oral antioxidants (N-acetyl-cysteine or vitamins A plus E) and essential fatty acids (FA) on sperm biology in an open prospective study including 27 infertile men. The evaluation included sperm characteristics, seminal reactive oxygen species (ROS), FA of sperm membrane phospholipids, sperm oxidized DNA (8-OH-dG), and induced acrosome reaction (AR). Treatment did not improve sperm motility and morphology, nor decrease the concentration of round cells and white blood cells in semen. Sperm concentration increased in oligozoospermic men (7.4 +/- 1.3 to 12.5 +/- 1.9 million/ml). Treatment significantily reduced ROS (mean +/- SEM) (775.3 +/- 372.2 to 150.3 +/- 105.2 x 10(3) counts/10 second) and 8-OH-dG (45.3 +/- 10.4 to 16.8 +/- 3.3 fmol/mug DNA). Treatment increased the AR (551 +/- 2.2 to 71.6 +/- 2.2 %), the proportion of polyunsaturated FA of the phospholipids, and sperm membrane fluidity. The overall pregnancy rate was 4.5% in 134 months. The per month pregnancy rate tended to be higher in partners of (ex)smokers (7.15%, n = 14, 70 months) than in never-smokers (1.6%, n = 13, 64 months) (OR:4.57, 95% Cl:0.55-38.1). (C) 2000 Harcourt Publishers Ltd.