LISTERIA-MONOCYTOGENES - SYNERGISTIC EFFECTS OF AMPICILLIN AND GENTAMICIN

作     者：AZIMI, PH KORANYI, K LINDSEY, KD 

作者机构：Division of Infectious Diseases Children’s Hospital Medical Center of Northern California Oakland California 

出 版 物：《AMERICAN JOURNAL OF CLINICAL PATHOLOGY》 (美国临床病理学杂志)

年 卷 期：1979年第72卷第6期

页      面：974-977页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

主　　题：氨苄西林/药理学 培养基 药物协同作用 庆大霉素类/药理学 单核细胞增生利斯特菌/药物作用 单核细胞增生利斯特菌/生长和发育 脑膜炎/血液 脑膜炎/脑脊髓液 脑膜炎/微生物学 儿童 人类 婴儿 新生 

摘      要：L. monocytogenes infections are most common in newborn infants and persons with impaired defense mechanisms. Although there are reports of successful treatment with ampicillin alone, there is uncertainty as to what regimen constitutes the most effective therapy. The in vitro synergism between ampicillin and gentamicin against L. monocytogenes was studied. Seven L. monocytogenes strains isolated from blood or CSF of infants and 3 control strains obtained from the Center for Disease Control were tested. Minimal inhibitory concentrations of ampicillin and gentamicin were determined in Todd-Hewitt broth with an inoculum of 105 organisms/ml. Killing curves were determined for ampicillin (6 .mu.g/ml), gentamicin (0.5 .mu.g/ml) and the combination of ampicillin (6 .mu.g/ml) plus gentamicin (0.5 .mu.g/ml). Incubation of approximately 107 organisms/ml with these concentrations of ampicillin and gentamicin caused no significant reduction in the viable bacterial population in 24 h. The combination was bactericidal in all 7 strains isolated from patients and 1 control strain. The ultimate test of the superiority of this combination to ampicillin alone probably must come from clinical studies. The synergistic and bactericidal effects of ampicillin with gentamicin may be desirable in treatment of newborns and patients with underlying disease.