Structural analysis of the catalytic and binding sites of <i>Clostridium botulinum</i> neurotoxin B

作     者：Swaminathan, S Eswaramoorthy, S 

作者机构：Brookhaven Natl Lab Dept Biol Upton NY 11973 USA 

出 版 物：《NATURE STRUCTURAL BIOLOGY》 (自然结构与分子生物学)

年 卷 期：2000年第7卷第8期

页      面：693-699页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：U.S. Department of Energy, USDOE Brookhaven National Laboratory, BNL 

主　　题：氨基酸序列 结合部位 肉毒毒素类/化学 肉毒毒素类/代谢 肉毒杆菌毒素类 A型/化学 肉毒杆菌毒素类 A型/代谢 催化域 肉毒梭菌/化学 结晶学 X线 神经节苷脂类/代谢 乳糖/化学 乳糖/代谢 模型 分子 分子序列数据 蛋白质结构 二级 蛋白质结构 三级 受体 细胞表面/化学 受体 细胞表面/代谢 序列比对 唾液酸类/化学 唾液酸类/代谢 

摘      要：Clostridium botulinum neurotoxins are among the most potent toxins to humans. The crystal structures of intact C. botulinum neurotoxin type B (BoNT/B) and its complex with sialyllactose, determined at 1.8 and 2.6 Angstrom resolution, respectively, provide insight into its catalytic and binding sites. The position of the belt region in BoNT/B is different from that in BoNT/A;this observation presents interesting possibilities for designing specific inhibitors that could be used to block the activity of this neurotoxin. The structures of BoNT/B and its complex with sialyllactose provide a detailed description of the active site and a model for interactions between the toxin and its cell surface receptor. The latter may provide valuable information for recombinant vaccine development.