Toxicity Effect of Cancer Cell Labeled with Visible Luminescent Nanocrystalline Silicon Particles and Visualization Observation in Vivo

作     者：Keisuke Sato Masaki Hiruoka Kohki Fujioka Naoki Fukata Kenji Hirakuri Kenji Yamamoto 

作者机构：SATO.Keisuke@nims.go.jp National Institute for Materials Scinece (NIMS) World Premier International (WPI) Research Center International Center for Materials Nanoarchitectonics (MANA) Tsukuba Japan mhiruoka@ff.f.dendai.ac.jp Tokyo Denki University Department of Electronic and Computer Engineering Hikigun Saitama Japan kfujioka@ri.imcj.go.jp International Medical Center of Japan Department of Medical Ecology and Informatics Shinjuku Tokyo Japan FUKATA.Naoki@nims.go.jp National Institute for Materials Scinece (NIMS) World Premier International (WPI) Research Center International Center for Materials Nanoarchitectonics (MANA) Tsukuba Ibaraki Japan hirakuri@eee.dendai.ac.jp Tokyo Denki University Department of Electronic and Computer Engineering Hikigun Saitama Japan backen@tcn-catv.ne.jp International Medical Center of Japan Department of Medical Ecology and Informatics Shinjuku Tokyo Japan 

出 版 物：《MRS Online Proceedings Library (OPL)》 

年 卷 期：2011年第1145卷第1期

页      面：1145-MM04-23-1145-MM04-23页

主　　题：biomaterial luminescence nanostructure 

摘      要：Cytotoxicity of human cervical carcinoma cell line (HeLa cells) labeled with the nanocrystalline silicon (nc-Si) particles before and after ultraviolet (UV) light exposure has studied on the viability and cellular membrane damages. The viability and cellular membrane damages of HeLa cells changed at high particle concentration of 1.12 mg/ml. The viability of HeLa cells labeled with the UV-exposed nc-Si particles was higher than that of unexposed nc-Si particles. However, the variation of cellular membrane damages was almost same for the nc-Si particles before and after UV exposure. These results substantiated the low toxicity of nc-Si particles. Moreover, the HeLa cells labeled with the nc-Si particles exhibited green fluorescence. On the other hand, in vivo test of nc-Si particles estimated by the visualization observation of the circulation from the lymphatic vessel to the lymph node of a mouse. The transfer pathway of nc-Si particles could be clearly monitored by the strong emission of red light.