Isolation and Characterization of a Curcin Promoter from Jatropha curcas L. and Its Regulation of Gene Expression in Transgenic Tobacco Plants

作     者：Qin, Xiaobo Zhang, Jinping Shao, Caixia Lin, Sha Jiang, Luding Zhang, Shuwen Xu, Ying Chen, Fang 

作者机构：Sichuan Univ Coll Life Sci Chengdu 610064 Peoples R China 

出 版 物：《PLANT MOLECULAR BIOLOGY REPORTER》 (植物分子生物学导报)

年 卷 期：2009年第27卷第3期

页      面：275-281页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：National Natural Science Foundation of China China International Science and Technology Cooperation Project [2004DFB00300, 2006DFB63400] Chinese Ministry of Education 

主　　题：Curcin Promoter Jatropha curcas L. Ribosome-inactivating protein 

摘      要：Ribosome-inactivating proteins (RIPs) represent those proteins that universally depurinate conserved alpha-sarcin loops of large rRNAs. In this study, a 0.6-kb fragment of a 5 flanking region preceding a curcin gene, encoding a type I RIP curcin, of Jatropha curcas L. endosperm was cloned, and its regulation of expression of the beta-glucuronidase (GUS) reporter gene was investigated in transgenic tobacco. Analysis of GUS activities showed that the 0.6-kb flanking fragment of the curcin gene was sufficient to drive the GUS reporter gene expression in tobacco seed. The activity of this flanking fragment was analyzed at different stages of seed development. Histochemical localization of GUS activity indicated that the promoter was specifically active in the endosperm tissue of the dicotyledonous tobacco embryo. Moreover, this activity was first initiated at the heart-shaped embryonic stage during seed development.