Chemical profile, toxicity, anti-acetylcholinesterase and antimicrobial activity of essential oil from hyptis dilatata leaves

作     者：Almeida, Sirley P. de Filho, Antonio Alves M. Simplicio, Fernanda Guilhom Martins, Regildo M. G. Takahashi, Jacqueline A. Ferraz, Vany P. de Melo, Ana Cristina G. R. Tadei, Wanderli P. 

作者机构：Post graduate in Biodiversity and Biotechnology Program PPG BIONORTE State Coordination of Amazonas ManausAmazonas Brazil Postgraduate Program in Chemistry PPGQ Center for Research and Graduate Studies in Science and Technology NPPGCT Departament of Chemistry and Postgraduate in Biotechnology and Biodiversity Program Rede Bionorte Federal University of Roraima Paricarana Campus Boa Vista-RRCEP 69304-000 Brazil Laboratory of Phytochemistry and Semi synthesis Faculty of Pharmaceutical Sciences Federal University of Amazonas Manaus AmazonasCEP 69077-000 Brazil Federal University of Pernambuco Immuno Pathology Laboratory Keizo-Ami Recife-PE Brazil Department of Chemistry Institute of Exact Sciences Universidade Federal de Minas Gerais CEP 31270-901 Belo HorizonteMinas Gerais Brazil Chromatography Laboratory Institute of Exact Sciences Federal University of Minas Gerais CEP 31270-901 Belo HorizonteMinas Gerais Brazil Post graduate in Natural Resources Program Federal University of Roraima Boa Vista-RR Brazil National Institute for Amazonian Research Manaus AmazonasCEP 69067-375 Brazil 

出 版 物：《Chemical Engineering Transactions》 (Chem. Eng. Trans.)

年 卷 期：2018年第64卷

页      面：271-276页

核心收录：

基　　金：To CNPq  FAPEMIG and FAPEAM for scholarships and financial support 

主　　题：Essential oils 

摘      要：The aim of this study was to evaluate the influence of seasonality on the chemical composition of the essential oil from Hyptis dilatata leaves, to perform biological activity assays such as antimicrobial, inhibition of acetylcholinesterase enzyme and to evaluate the toxicity of the essential oil using Artemia salina as indicator on the test. Hyptis dilatata leaves were collected in rainy and dry seasons, in the morning, in the afternoon and in the evening. It was extracted by hydrodistillation using the extractor of Clevenger condenser double Spell model. The essential oil analysis resulted in 22 chemical components. The major constituents of the dry and rainy periods were a-pinene (18.7% in the night and 12.9% in the afternoon), 3-carene (26.5% and 19.9% in the night), fenchone (43% and 33.7% in the morning) and ß-caryophyllene 9.1% in the afternoon and 6.1% in the morning. The essential oil in vitro inhibited the acetylcholinesterase enzyme in 99.9% in the afternoon (rainy period) and 96.4% in the morning (dry period). Between the dry and rainy seasons, the lowest LC50 microbial activity in vitro was obtained for leaves collected during rainy season, in the morning period tested against the bacterium Staphylococcus aureus (LC50 78.1 mg mL-1). The cytotoxic activity of the essential oil of H. dilatata on Artemia salina showed LC50 results below 100 µg mL-1 and in the afternoon during the rainy period and at night in the dry period the results are above this value. Therefore, the chemical characterization and biological activities tests of essential oils showed promising results in the search for new active substances and the development of bioproducts of vegetable origin. Copyright © 2018, AIDIC Servizi S.r.l.