Evaluation of calcium-alginate gel as an artificial diet medium for bioassays on common cutworms

作     者：Morimoto, M Matsuda, K Ohta, Y Ihara, T Komai, K 

作者机构：Kinki Univ Dept Agr Chem Nara 6318505 Japan 

出 版 物：《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 (J. Agric. Food Chem.)

年 卷 期：2004年第52卷第15期

页      面：4737-4739页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 09[农学] 0703[理学-化学] 0901[农学-作物学] 

主　　题：bioassay method calcium-alginate gel artificial diet insecticidal compounds antifeedants lepidopteran larvae 

摘      要：A calcium-alginate gel diet was developed for Spodoptera litura larvae, and its reliability as a carrier for incorporating antifeedants as well as insecticides was investigated. The alginate gel diet was prepared with a simple protocol, which does not involve any heating process. When tested using this diet, acephate, a Bacillus thuringiensis endotoxin formulation and rotenone reproducibly showed insecticidal activity against the larvae, while neem oil and scabequinone deterred the larval feeding effectively. However, not only the insecticidal activity of acephate but also the antifeedant activity of neem oil was reduced by replacing the alginate component by agar in the diet, suggesting the usefulness of the alginate gel diet as an assay tool for testing a broad range of samples against the larvae.