<i>XIST</i> RNA associates with specific regions of the inactive X chromatin

作     者：Gilbert, SL Pehrson, JR Sharp, PA 

作者机构：MIT Ctr Canc Res Dept Biol Cambridge MA 02139 USA Univ Penn Sch Vet Med Dept Biol Anim Philadelphia PA 19104 USA 

出 版 物：《JOURNAL OF BIOLOGICAL CHEMISTRY》 (生物化学杂志)

年 卷 期：2000年第275卷第47期

页      面：36491-36494页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：NCI NIH HHS [P30-CA14051] Funding Source: Medline NIGMS NIH HHS [R37-GM34277] Funding Source: Medline 

主　　题：抗体特异性 细胞 培养的 染色质/化学 剂量补偿作用 遗传学 纤连蛋白类/遗传学 组蛋白类/遗传学 免疫血清 启动区(遗传学) RNA 未翻译/遗传学 RNA 未翻译/免疫学 转录因子/遗传学 转录因子/免疫学 X染色体 动物 小鼠 

摘      要：Microscopy studies have shown that XIST RNA colocalizes with the inactive X chromosome (Xi). However, the molecular basis for this colocalization is unknown, Here we provide two lines of evidence from chromatin immunoprecipitation experiments that XTST RNA physically associates with the Xi chromatin. First, XTST RNA can be co-precipitated by antiserum against macroH2A, a histone H2A variant enriched in the Xi. Second, XIST RNA can be co-precipitated by antisera that recognize unacetylated, but not acetylated, isoforms of histones H3 and H4. The specificity of XIST RNA association with hypoacetylated chromatin, together with the previous finding that hypoacetylated histone H4 is enriched at promoters of X-inactivated genes, raises the possibility that XIST RNA may contribute to the hypoacetylation of specific regions of the Xi so as to alter the expression of X-linked genes.