PRODUCTION OF AUTO-ANTI-IDIOTYPIC ANTIBODY DURING THE NORMAL IMMUNE-RESPONSE .12. AN ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR AUTO-ANTI-IDIOTYPE ANTIBODY

作     者：GIBBONS, JJ GOIDL, EA SHEPHERD, GM THORBECKE, GJ SISKIND, GW 

作者机构：CORNELL UNIV MED CTR COLL MED DEPT MED DIV ALLERGY & IMMUNOL NEW YORK NY 10021 USA NYU SCH MED DEPT PATHOL NEW YORK NY 10016 USA 

出 版 物：《JOURNAL OF IMMUNOLOGICAL METHODS》 (免疫法杂志)

年 卷 期：1985年第79卷第2期

页      面：231-237页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：NIAID NIH HHS [AI-11694] Funding Source: Medline NIA NIH HHS [AG-04060] Funding Source: Medline 

主　　题：auto-anti-idiotype idiotype ELISA for anti-idiotype BGG bovine gamma globulin BSA bovine serum albumin F Ficoll Id idiotype Ig immunoglobulin PFC plaque-forming cell(s) TNP 2,4,6-trinitrophenyl group TNP-F trinitrophenyllysyl-Ficoll 

摘      要：An enzyme-linked immunosorbent assay (ELISA) to detect anti-idiotype (Id) antibody was described. Using this assay auto-anti-Id was detected in the serum of aged mice immunized with 2,4,6-trinitrophenylated-Ficoll (TNP-F). Hapten eluates from anti-TNP-F immune spleen cells also contained readily detectable auto-anti-Id.