A polypyrrole protein microarray for antibody-antigen interaction studies using a label-free detection process

作     者：Grosjean, L Cherif, B Mercey, E Roget, A Levy, Y Marche, PN Villiers, MB Livache, T 

作者机构：Univ Grenoble 1 INSERM U548 Lab Immunochim CEA GDRDC F-38054 Grenoble France UJF CNRS CEADRFMC CREBUMR 5819 F-38054 Grenoble France Inst Opt Orsay LCFIO F-91043 Orsay France 

出 版 物：《ANALYTICAL BIOCHEMISTRY》 (分析生物化学)

年 卷 期：2005年第347卷第2期

页      面：193-200页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 0703[理学-化学] 

基　　金：California Earthquake Authority, CEA Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, MESRS Ministère des relations internationales et de la Francophonie, MRIF 

主　　题：protein microarray polypyrrole surface plasmon resonance imaging antigen antibody 

摘      要：Protein microarray is a promising technology that should combine rapidity and easy use with high throughput and versatility. This article describes a method in which an electrocopolymerization process is employed to graft biological molecules on to a chip so that surface plasmon resonance imaging may be used to detect molecular interactions. Copolymerization of pyrrole-modified protein and pyrrole is an efficient grafting process which immobilizes molecules at defined positions on a gold surface. Surface plasmon resonance imaging is an optical technique that allows real-time simultaneous detection of molecular interactions on a large number of spots without labeling. This method was successfully used to analyze antibody-antigen interactions. This illustrates its high specificity and good sensitivity and demonstrates its suitability for biological studies. (c) 2005 Elsevier Inc. All rights reserved.