Comparison of the attachment of <i>Escherichia coli</i> O157:H7, <i>Listeria monocytogenes</i>, <i>Salmonella typhimurium</i>, and <i>Pseudomonas fluorescens</i> to lettuce leaves

作     者：Takeuchi, K Matute, CM Hassan, AN Frank, JF 

作者机构：Univ Georgia Dept Food Sci & Technol Ctr Food Safety & Qual Enhancement Athens GA 30602 USA 

出 版 物：《JOURNAL OF FOOD PROTECTION》 (食品保护杂志)

年 卷 期：2000年第63卷第10期

页      面：1433-1437页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 0836[工学-生物工程] 

主　　题：细菌黏附/生理学 细菌学技术 集落计数 微生物 大肠杆菌O157/生理学 莴苣/微生物学 单核细胞增生利斯特菌/生理学 显微镜检查 共焦/方法 荧光假单胞菌/生理学 鼠伤寒沙门菌/生理学 

摘      要：Attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas fluorescens on iceberg lettuce was evaluated by plate count and confocal scanning laser microscopy (CSLM). Attachment of each microorganism (similar to 10(8) CFU/ml) on the surface and the cut edge of lettuce leaves was determined. E. coli O157:H7 and L. monocytogenes attached preferentially to cut edges, while P. fluorescens attached preferentially to the intact surfaces. Differences in attachment at the two sites were greatest with L. monocytogenes. Salmonella Typhimurium attached equally to the two sites. At the surface, P. fluorescens attached in greatest number, followed by E. coli O157:H7, L. monocytogenes, and Salmonella Typhimurium. Attached microorganisms on lettuce were stained with fluorescein isothiocyanate and visualized by CSLM. Images at the surface and the cut edge of lettuce confirmed the plate count data. In addition, microcolony formation by P. fluorescens was observed on the lettuce surface. Some cells of each microorganism at the cut edge were located within the lettuce tissues, indicating that penetration occurred from the cut edge surface. The results of this study indicate that different species of microorganisms attach differently to lettuce structures, and CSLM can be successfully used to detect these differences.