Construction of a linkage map of Lentinula edodes (shiitake) with the HEGS (high-efficiency genome scanning) system: Use of versatile AFLP and PCR-based gene markers

作     者：Terashima, Kazuhisa Matsumoto, Teruyuki Hayashi, Eiji Kawasaki, Shinji Fukumasa-Nakai, Yukitaka 

作者机构：Japan Science and Technology Corporation (JST) Saitama Japan Tottori Mycological Institute Tottori 689-1125 211 Kokoge Japan California State Polytechnic University Pomona CA United States National Institute of Agrobiological Resources Tsukuba Ibaraki Japan 

出 版 物：《Mycoscience》 (Mycoscience)

年 卷 期：2006年第47卷第6期

页      面：336-346页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 0713[理学-生态学] 10[医学] 

主　　题：Breeding High-efficiency genome scanning system Linkage map Shiitake 

摘      要：An improved linkage map of Lentinula edodes (shiitake) was constructed with an HEGS (high-efficiency genome scanning) system. Two hundred twenty-one HEGS-derived amplified fragment length polymorphism (AFLP-H) markers and 21 gene markers were developed and combined with 203 previously developed sequencer-derived AFLP markers (AFLP-S markers) and 3 mating factor loci (A, Bα, and Bβ) to construct a comprehensive linkage analysis. As a result, a novel linkage map with 166 markers including 2 mating factors (A and B), 10 HEGS-derived gene markers, 72 AFLP-H markers, and 82 AFLP-S markers was obtained. Of the total 448 markers, 273 could not be located on a linear map and thus were assigned to linkage groups as accessory markers. The map covers a total length of 1398.4 centimorgans (cM) with an average marker interval distance of 8.4cM. The map consists of 11 linkage groups (LGs) in agreement with our previous map, and 7 LGs among them were found to contain branched linkages, which may be the result of reciprocal translocations representing dynamic reorganization of the shiitake genome. The previously reported linkage map was improved in terms of number of markers, marker density, linear order of markers, and total map length. © 2006 The Mycological Society of Japan and Springer-Verlag Tokyo.