DNA microarray-based detection and identification of bacterial and viral pathogens of maize

作     者：Krawczyk, Krzysztof Uszczynska-Ratajczak, Barbara Majewska, Alicja Borodynko-Filas, Natasza 

作者机构：Natl Res Inst Inst Plant Protect Virol & Bacteriol Dept Wladyslawa Wegorka 20 PL-60318 Poznan Poland Ctr Genom Regulat Dr Aiguader 88 Barcelona 08003 Catalonia Spain UPF Barcelona Spain Warsaw Univ Life Sci Dept Physiol Sci Fac Vet Med Nowoursynowska 159C PL-02776 Warsaw Poland 

出 版 物：《JOURNAL OF PLANT DISEASES AND PROTECTION》 (J. Plant Dis. Prot.)

年 卷 期：2017年第124卷第6期

页      面：577-583页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 

基　　金：National Center of Science [NN310118237  NN310085436  2011/01/N/NZ9/07091  DEC-2011/01/N/NZ9/07091] 

主　　题：DNA microarray Maize pathogen detection Pantoea ananatis P. agglomerans Enterobacter cloaceae subsp dissolvens Maize dwarf mosaic virus (MDMV) Sugarcane mosaic virus (SCMV) 

摘      要：We describe the construction and evaluation of DNA microarray for simultaneous detection and identification of five microbial pathogens of maize: Pantoea ananatis, P. agglomerans, Enterobacter cloaceae subsp. dissolvens, Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV). Unlike other DNA microarrays described, our microarray comprises probes targeting the whole genomes of the tested pathogens. Control probes are complementary to genomes of closely related microorganisms that are nonpathogenic to maize and against maize and human genome sequences in order to avoid the potential false-positive results. Obtained results indicate that the fluorescence signals from pathogen and control probes are well distinguished in all performed experiments. The microarray s performance was compared with classical PCR-based pathogen detection method, and the versatility of the assay was tested in silico.