Effect of conjugated bile salts on antibiotic susceptibility of bile salt-tolerant <i>Lactobacillus</i> and <i>Bifidobacterium</i> isolates

作     者：Charteris, WP Kelly, PM Morelli, L Collins, JK 

作者机构：SET Consultants Ltd Cork Ireland Natl Dairy Prod Res Ctr Cork Ireland Univ Cattolica Sacro Cuore Inst Microbiol Piacenza Italy Univ Coll Dept Microbiol Cork Ireland 

出 版 物：《JOURNAL OF FOOD PROTECTION》 (食品保护杂志)

年 卷 期：2000年第63卷第10期

页      面：1369-1376页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：Key Laboratory of Mineralogy and Metallogeny of the Chinese Academy of Sciences at Guangzhou Institute of Geochemistry(KLMM20150103) State Key Laboratory of Heavy Oil National Natural Science Foundation of China(1103312, 1210040, 21103045) China University of Petroleum, Beijing(SKCHOP201504) 

主　　题：抗菌药/药理学 二裂菌属/药物作用 二裂菌属/生长和发育 二裂菌属/分离和提纯 胆汁酸类和盐类/药理学 消化系统/微生物学 抗药性 微生物 乳杆菌属/药物作用 乳杆菌属/生长和发育 乳杆菌属/分离和提纯 微生物敏感性试验 有益菌种 泌尿生殖系统/微生物学 女(雌)性 人类 男(雄)性 

摘      要：Virtually every antibiotic may cause in vivo alterations in the number, level, and composition of the indigenous microbiotae. The degree to which the microbiotae are disturbed depends on many factors. Although bile may augment antibiotic activity, studies on the effect of bile on the antibiotic susceptibility of indigenous and exogenous probiotic microorganisms are lacking. It was against this background that the antibiotic susceptibility of 37 bile salt-tolerant Lactobacillus and 11 Bifidobacterium isolates from human and other sources was determined in the presence of 0.5% wt/wt oxgall (conjugated bile salts). Oxgall did not affect the intrinsic resistance of lactobacilli to metronidazole (5 mu g), vancomycin (30 mu g), and cotrimoxazole (25 mu g), whereas it resulted in a complete loss of resistance to polymyxin B (300 mu g) and the aminoglycosides gentamicin (10 mu g), kanamycin (30 mu g), and streptomycin (10 mu g) for most strains studied (P 0.001). Oxgall did not affect the intrinsic resistance of bifidobacteria to metronidazole and vancomycin, whereas polymyxin B and co-trimoxazole resistance was diminished (P 0.05) and aminoglycoside resistance was lost (P ( 0.001). Seven lactobacilli, but no bifidobacteria strain, showed unaltered intrinsic antibiotic resistance profiles in the presence of oxgall. Oxgall affected the extrinsic susceptibility of lactobacilli and bifidobacteria to penicillin G (10 mu g), ampicillin (10 mu g). tetracycline (30 mu g), chloramphenicol (30 mu g), erythromycin (15 mu g), and rifampicin (5 mu g) in a source- and strain-dependent manner. Human strain-drug combinations of lactobacilli (P 0.05) and bifidobacteria (P 0.01) were more likely to show no change or decreased susceptibility compared with other strain-drug combinations. The antimicrobial activity spectra of polymyxin B and the aminoglycosides should not be considered limited to gram-negative bacteria but extended to include gram-positive genera of the indigenous and