Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis

作     者：邓思平 陈松林 刘本伟 DENG Si-ping;CHEN Song-lin;LIU Ben-wei

作者机构：广东海洋大学水产学院广东湛江*** 中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2008年第29卷第6期

页      面：592-598页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by grants from State 863 High-Technology R&D Project of China(2006AA10A403) Shandong Genetic Improvement Key Project for Agricultural Organism Doctor Initial Funding of Guangdong Ocean University(0712103) 

主　　题：Cynoglossus semilaevis FTZ-F1 cDNA cloning Expression 

摘      要：To investigate the expression characteristics of sex related gene of FTZ-F 1 in the half-smooth tongue-sole （Cynoglossus semilaevis）, the homologue FTZ-F1 （hsFTZ-F1） full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66bp 5＇-UTR, along with a 1619bp 3＇-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.