Isolation,Identification,and Characterization of an Aluminum-Tolerant Bacterium Burkholderia ***1 from an Acidic Red Soil

作     者：HUANG Shoucheng WANG Xiaodong LIU Xu HE Genhe WU Jichun 

作者机构：School of Life SciencesKey Laboratory of Biology Diversity and Ecological Engineering of Jiangxi ProvinceJinggangshan UniversityJi'an 343009(China) School of Life SciencesAnhui Science and Technology UnzversityFengyang 233100(China) Jiangxi Academy of ForestryNanchang 330013(China) Department of HydrosciencesState Key Laboratory of Pollution Control and Resources ReuseNanjing UniversityNanjing 210093(China) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2018年第28卷第6期

页      面：905-912页

核心收录：

学科分类：07[理学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (No. 41462008) the Ph.D. Research Startup Foundation of Jinggangshan University, China (No. JZB1307) 

主　　题：antibiotics bioremediation Al resistance Al toxicity microbial community plant growth-promoting bacteria temperature 

摘      要：Aluminum(Al) toxicity usually occurs in acidic soils worldwide, which is detrimental to the growth of organisms. An Al-tolerant bacterium, SB1, was isolated from an acidic red soil of Chingkang Mountain, located in Jiangxi Province of China. Polyphasic analysis,including a 16 S rDNA phylogenetic tree as well as morphological and physicochemical properties, revealed that the isolate was a gramnegative, rod-shaped bacterium, which was recognized as Burkholderia sp. SB1 and had extreme acidity tolerance(pH 2.2) and excellent Al resistance(270 mg L^(-1) Al^(3+)). It could remove Al by up to 97.7% at a concentration of 54 mg L^(-1) Al^(3+). SB1 behavior under different temperatures and antibiotics was also examined. SB1 preferred moderate temperature conditions, ranging from 25 to37?C, and exhibited notable resistance to multiple antibiotics(including ampicillin, streptomycin, and tetracycline), except for being sensitive to chloramphenicol. Therefore, as the first reported bacterium to possess favorable Al resistance and excellent Al removal,Burkholderia sp. SB1 can potentially be used as an agent for bioremediation of Al-contaminated acidic red soils.