Systematic evaluation and optimization of crystallization conditions for vancomycin purification

作     者：Lee, Ji-Yeon Lee, Kang-Hee Chae, Hee-Jeong Kim, Jin-Hyun 

作者机构：Kongju Natl Univ Dept Chem Engn Kong Ju 314701 South Korea Hoseo Univ Dept Food & Biotechnol Asan 336795 South Korea 

出 版 物：《KOREAN JOURNAL OF CHEMICAL ENGINEERING》 (韩国化工杂志)

年 卷 期：2010年第27卷第5期

页      面：1538-1546页

核心收录：

学科分类：081704[工学-应用化学] 0817[工学-化学工程与技术] 08[工学] 0703[理学-化学] 

主　　题：Vancomycin Crystallization Purification Optimization of Process Parameters Identification of Morphology 

摘      要：This study describes the evaluation and optimization of a crystallizing process capable of efficiently purifying vancomycin in high purity and yield. In particular, we observed how the main process parameters influenced the formation of crystals, determined their morphology, and monitored purity and yield. Acetone was shown to be more effective than alcohol solvents for the crystallization of vancomycin. The optimal distilled water/acetone ratio, storage temperature, storage time, pH, conductivity, initial vancomycin concentration and stirrer velocity were shown to be 1:3.5 (v/v), 10 degrees C, 24 h, pH 2.5, 20 ms/cm, 0.1 g/mL, and 640 rpm, respectively. Temperature had a decisive influence on crystal formation;crystals were successfully produced at 10 degrees C, while at other temperatures, conglomeration, disintegration and cohesion occurred. Crystal growth developed over time and was complete at about 24 h. Vancomycin purity remained at about 97.0% irrespective of storage time while the yield increased over time, reaching a maximum of 95.0% at around 24 h, after which there was no substantial change. Crystallization occurred over a certain range of pH (2.5-3.0), but purity and yield were highest at pH 2.5. When the pH was outside this range, a conglomeration (gelation) phenomenon prevented the efficient production of crystals. Vancomycin crystals were produced irrespective of the stirrer velocity, which had no influence on purity;however, the highest yield of vancomycin was obtained at 640 rpm.