Defects in nuclear and cytoskeletal morphology and mitochondrial localization in spermatozoa of mice lacking nectin-2, a component of cell-cell adherens junctions

作     者：Bouchard, MJ Dong, YZ McDermott, BM Lam, DH Brown, KR Shelanski, M Bellvé, AR Racaniello, VR 

作者机构：Columbia Univ Coll Phys & Surg Dept Microbiol New York NY 10032 USA Columbia Univ Coll Phys & Surg Dept Pathol New York NY 10032 USA Columbia Univ Coll Phys & Surg Dept Anat & Cell Biol New York NY 10032 USA Columbia Univ Coll Phys & Surg Dept Urol New York NY 10032 USA Columbia Univ Coll Phys & Surg Ctr Reprod Sci New York NY 10032 USA 

出 版 物：《MOLECULAR AND CELLULAR BIOLOGY》 (分子生物学与细胞生物学)

年 卷 期：2000年第20卷第8期

页      面：2865-2873页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：NIAID NIH HHS [AI34418] Funding Source: Medline 

主　　题：细胞黏附分子/生理学 细胞核/病理学 细胞核/生理学 细胞核/超微结构 细胞骨架/病理学 细胞骨架/生理学 细胞骨架/超微结构 基因表达调控/生理学 细胞连接/遗传学 精子/病理学 精子/生理学 精子/超微结构 动物 人类 男(雄)性 小鼠 大鼠 

摘      要：Nectin-2 is a cell adhesion molecule encoded by a member of the poliovirus receptor gene family. This family consists of human, monkey, rat, and murine genes that are members of the immunoglobulin gene superfamily, Nectin-2 is a component of cell-cell adherens junctions and interacts with 1-afadin, an F-actin-binding protein, Disruption of both alleles of the murine nectin-2 gene resulted in morphologically aberrant spermatozoa with defects in nuclear and cytoskeletal morphology and mitochondrial localization. Homozygous null males are sterile, while homozygous null females, as well as heterozygous males and females, are fertile, The production by nectin-2(-/-) mice of normal numbers of spermatozoa containing wild-type levels of DNA suggests that Nectin-2 functions at a late stage of germ cell development. Consistent with such a role, Nectin-2 is expressed in the testes only during the later stages of spermatogenesis. The structural defects observed in spermatozoa of nectin-2(-/-) mice suggest a role for this protein in organization and reorganization of the cytoskeleton during spermiogenesis.