Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

作     者：Liu, Dazhi Wei, Yifeng Liu, Xuyang Zhou, Yan Jiang, Li Yin, Jinyu Wang, Feifei Hu, Yiling Urs, Ankanahalli N. Nanjaraj Liu, Yanhong Ang, Ee Lui Zhao, Suwen Zhao, Huimin Zhang, Yan 

作者机构：Tianjin Univ Sch Pharmaceut Sci & Technol Collaborat Innovat Ctr Chem Sci & Engn Tianjin Key Lab Modern Drug Delivery & High Effic Tianjin 300072 Peoples R China ASTAR Inst Chem & Engn Sci Metab Engn Res Lab Singapore 138669 Singapore ShanghaiTech Univ iHuman Inst Shanghai 201210 Peoples R China ShanghaiTech Univ Sch Life Sci & Technol Shanghai 201202 Peoples R China Chinese Acad Sci Tech Inst Phys & Chem Beijing 100190 Peoples R China Univ Illinois Dept Chem & Biomol Engn 600 South Mathews Ave Urbana IL 61801 USA 

出 版 物：《NATURE COMMUNICATIONS》 (Nat. Commun.)

年 卷 期：2018年第9卷第1期

页      面：4224页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：National Science Foundation of China Agency for Science, Research and Technology of Singapore Visiting Investigator Program ShanghaiTech University Shanghai Municipal Government 

主　　题：Biochemistry Computational biology and bioinformatics 

摘      要：Skatole is a malodorous compound that contributes to the characteristic smell of animal faeces. Although skatole has long been known to originate from bacterial tryptophan fermentation, the enzyme catalysing its formation has so far remained elusive. Here we report the use of comparative genomics for the discovery of indoleacetate decarboxylase, an O-2-sensitive glycyl radical enzyme catalysing the decarboxylation of indoleacetate to form skatole as the terminal step of tryptophan fermentation in certain anaerobic bacteria. We describe its biochemical characterization and compare it to other glycyl radical decarboxylases. Indoleacetate decarboxylase may serve as a genetic marker for the identification of skatole-producing environmental and human-associated bacteria, with impacts on human health and the livestock industry.