Isolation of a Pseudomonas Stutzeri strain that degrades 1,2,4-trichlorobenzene and characterization of its degradative plasmid

作     者：Lei SONG Hui WANG Hanchang SHI Hongying HU 

作者机构：Environmental Simulation and Pollution Control State Key Joint LaboratoryDepartment of Environmental Science and EngineeringTsinghua UniversityBeijing 100084China 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2008年第2卷第1期

页      面：69-72页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：This work was supported by the National High Technology Research and Development Program of China(863 Program)(Grant Nos.2002AA601170 and 2002A A601150) 

主　　题：1,2,4-trichlorobenzene 16S rDNA degradative plasmid Pseudomonas sp. 

摘      要：The genetic information encoding metabolic pathways for xenobiotic compounds in bacteria often resides on catabolic *** aim of the present work was to know the location of the genes for degrading 1,2,*** this paper a 1,2,4-trichlorobenzene-degrading strain THSL-1 was isolated from the soil of Tianjin Chemical Plant using 1,2,4-trichlorobenzene as the sole carbon *** strain was identified as Pseudomonas stutzeri through morphologic survey and 16S rDNA sequence determination.A plasmid was discovered from strain THSL-1 by using the alkali lysis *** the plasmid was transformed into ***109 by the CaCl2 method,the transformant could grow using 1,2,4-trichlorobenzene as the sole carbon source and had the degradation function of 1,2,***,it could be deemed that the plasmid carried the degradative genes of 1,2,*** average size of the plasmid was finally determined to be 40.2 Kb using selectively three kinds of restricted inscribed enzymes(HindIII,BamHI,and XholI)for single cutting and double cutting the plasmid pTHSL-1,respectively.