TRIACYLGLYCEROL LIPASE ACTIVITY IN BAKERS-YEAST (SACCHAROMYCES-CEREVISIAE)

作     者：SCHOUSBOE, I 

作者机构：UNIV COPENHAGEN PANUM INST DEPT BIOCHEM C BLEGDAMSVEJ 3 DK-2200 COPENHAGEN DENMARK 

出 版 物：《BIOCHIMICA ET BIOPHYSICA ACTA》 (生物化学与生物物理学报 - 血脂和脂质代谢)

年 卷 期：1976年第424卷第3期

页      面：366-375页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：Danish State Science Research Council 

摘      要：An investigation of intracellular triacylglycerol lipase activity in baker s yeast (Saccharomyces cerevisiae) has been performed using emulsified triolein as substrate. Bovine serum has been used as emulsifier since it was found superior to gum arabic and albumin with respect to reproducibility of both triacylglycerol concentration in the assay mixture and specific lipase activity. No extracellular activity could be detected neither with whole cells nor with water or detergent “extracts of intact cells as enzyme source. With disrupted cells the level of triacylglycerol lipase activity at a triacylglycerol concentration of 9.6 mM, at pH 7.5, and 30° C was 190 μmol free fatty acids per h per g disrupted cells. Fractionation of a cytoplasmic extract of disrupted cells revealed that about 70% of the activity was associated with membrane fractions and 60% of this activity was present in the mitochondrial fraction. Purification of this fraction was followed by an increase in specific lipase activity which parallels the increase in specific activity of the cytochrome c oxidase.