DETERMINATION OF OPTIMAL INVITRO CONDITIONS FOR CAPRINE ALTERNATIVE COMPLEMENT PATHWAY ASSAY

作     者：VENUGOPAL, G RAM, GC BANSAL, MP 

作者机构：INDIAN VET RES INSTIMMUNOL SECTIZATNAGAR 243122UTTAR PRADESHINDIA 

出 版 物：《VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY》 (兽医免疫学和免疫病理学)

年 卷 期：1992年第32卷第3-4期

页      面：359-364页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 0906[农学-兽医学] 09[农学] 

主　　题：ACP alternative complement pathway C3 third component of complement GVBS VBS containing 10 mM EDTA plus 0.1% gelatin (pH 7.4) VBS veronal buffered saline VBS-EGTA 2+ VBS containing 10 mM EGTA and 10 mM MgCl 2 (pH 7.4) 

摘      要：Optimal in vitro testing conditions for caprine alternative complement pathway assay were determined. Effects of the following variables were tested: heterologous erythrocytes;pH, ionic strength and Mg2+ ion concentration of the complement diluent;incubation time and temperature. Rabbit erythrocytes were the optimal target cells. The optimal buffer conditions were: pH 8.0, ionic strength 0.06 mmol NaCl and 5mmol Mg2+ ion. Optimal incubation time and temperature were 75 min and 30-degrees-C, respectively.