A bioseparation apparatus with high-pressure fluid injection and fluid sampling

作     者：Dreier, GH Tao, F Hess, RA Cheung, CY Sciaba, LE Green, DJ Laugharn, JA 

作者机构：BMA Labs Inc Unit F Woburn MA 01801 USA BioSeq Inc Unit F Woburn MA 01801 USA 

出 版 物：《ANALYTICAL BIOCHEMISTRY》 (分析生物化学)

年 卷 期：1999年第269卷第2期

页      面：223-229页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 0703[理学-化学] 

基　　金：NIGMS NIH HHS [F32 GM017734] Funding Source: Medline 

主　　题：抗体/分离和提纯 细菌噬菌体λ/化学 生物化学/仪器和设备 缓冲剂 色谱法 亲和 色谱法 离子交换 DNA 病毒/分离和提纯 流体静力压 RNA/分离和提纯 动物 

摘      要：A novel apparatus in which fluids may be injected and sampled at high pressure is described. Bioseparation applications of the apparatus were demonstrated in three model systems: (1) lambda DNA was eluted under pressure from an anion exchange column into a low-salt (0.25 M) buffer, thereby eliminating conventional time-consuming desalting procedures required for downstream analysis of the DNA;(2) RNA was separated under pressure from a RNA/DNA mixture, thereby enabling rapid differential preparation of nucleic acids;and (3) an antibody was purified from a protein mixture by affinity capture at one pressure and dissociation from the antigen binding partner at a second pressure, thereby enabling the immunoreactivities of both antibody and antigen to be preserved during the separation process. (C) 1999 Academic Press.