Characterization of hPRP4 kinase activation: potential role in signaling

作     者：Huang, Y Deng, TL Winston, BW 

作者机构：Univ Calgary Dept Med Calgary AB T2N 4N1 Canada Univ Calgary Dept Biochem & Mol Biol Div Crit Care Med Calgary AB T2N 4N1 Canada Univ Calgary Immunol Res Grp Calgary AB T2N 4N1 Canada Univ Florida Coll Med Dept Biochem & Mol Biol Gainesville FL 32610 USA 

出 版 物：《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》 (生物化学与生物物理学研究通讯)

年 卷 期：2000年第271卷第2期

页      面：456-463页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：Department of Medicine, University of Calgary Alberta Heritage Foundation for Medical Research, AHFMR Medical Research Council Canada, MRC 

主　　题：印迹法 RNA COS细胞 DNA结合蛋白质类 酶激活 表皮生长因子/代谢 福司柯林/代谢 基因文库 萤光素酶类/代谢 诱变 定点 磷酰化 沉淀素试验 蛋白质丝氨酸苏氨酸激酶/遗传学 蛋白质丝氨酸苏氨酸激酶/代谢 原癌基因蛋白质类/代谢 核糖核蛋白 U4-U6小核/遗传学 核糖核蛋白 U4-U6小核/代谢 信号传导 苏氨酸/代谢 组织分布 转录因子 转染 ets结构域蛋白Elk1 动物 人类 

摘      要：Mitogen-activated protein kinases (MAPKs) and cyclin-dependent kinases (CDKs) are important proline-directed Ser/Thr kinases that play distinct roles in cell differentiation and proliferation. hPRP4 (pre-mRNA processing gene), a human homologue of S. pombe Prp4, is a recently isolated CDK-like kinase with homology to MAPKs. Little is known about the mRNA processing function of hPRP4 or about the signaling pathways with which it is associated. hPRP4 is expressed in a variety of human tissues with the highest expression in the brain, lung and liver. In this paper, we characterize the activation of hPRP4 in COS-7 cells and show that hPRP4 also possesses a transcription factor activation function. hPRP4 is activated by epidermal growth factor (EGF) or forskolin treatment, but not tetradecanoyl phorbol acetate (TPA) nor ultraviolet (UV) irradiation. Activated hPRP4 phosphorylates residue Thr-417 on Elk-1 resulting in Elk-l activation. This site of Elk-1 phosphorylation is distinct from that of other MAPKs. Coexpression of hPRP4 with an Elk-1 reporter construct causes trans activation of the reporter. These findings suggest that hPRP4, a CDK-Like kinase related to MAPKs, may play a distinct role in signal transduction in ad dition to its role in mRNA processing. (C) 2000 Academic Press.