Improvement of detection method of Cryptosporidium and Giardia in reclaimed water

作     者：Tong ZHANG Xing XIE Hongying HU Yudong SONG Qianyuan WU Zusheng ZONG 

作者机构：Environmental Simulation and Pollution Control State Key Joint LaboratoryDepartment of Environmental Science and EngineeringTsinghua UniversityBeijing 100084China 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2008年第2卷第3期

页      面：380-384页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：This study was supported by the National Natural Science Foundation of China(Grant No.20510076) National Natural Science Foundation-Japan Science and Technology Agency Joint-Project(Grant No.20477021) 

主　　题：concentration Cryptosporidium Giardia immunomagnetic separation(IMS) wastewater reuse 

摘      要：Cryptosporidium and Giardia are two typical species of pathogenic protozoans that seriously endanger water *** works indicated that detection of Cryptosporidium and Giardia with modified United States Environmental Protection Agency(USEPA)method-1623 using a membrane filtration-elution for sample concentration attained better recovery and lower cost compared to the USEPA *** improvements of membrane filtration-elution step as well as immunomagnetic separation(IMS)steps were investigated and an optimized method for detection of Cryptosporidium and Giardia in wastewater reclamation system was recommended in this *** experimental results show that an overnight soak of the membrane after scraping and vortex agitation before elution could enhance and stabilize the *** turbidity to 4 NTU by adding kaolin clay before filtration could effectively improve the recovery of low-turbidity *** the concentrate after centrifugation and twice acid dissociation both reduced the impact of water quality to protozoan *** in different water samples were determined by this optimized method,and the recovery of Cryptosporidium and Giardia were above 70% and 80%respectively,much higher than the acceptance of method-1623.