Sensitive detection of multiple mycotoxins by SPRi with gold nanoparticles as signal amplification tags

作     者：Hu, Weihua Chen, Hongming Zhang, Hilanhuan He, Guangli Li, Xin Zhang, Xiaoxing Liu, Yang Li, Chang Ming 

作者机构：Southwest Univ Inst Clean Energy & Adv Mat Chongqing 400715 Peoples R China Chongqing Key Lab Adv Mat & Technol Clean Energie Chongqing 400715 Peoples R China Southwest Univ Fac Mat & Energy Chongqing 400715 Peoples R China Chongqing Univ State Key Lab Power Transmiss Equipment & Syst Se Chongqing 400044 Peoples R China Chinese Acad Agr Sci Inst Agroprod Proc Sci & Technol Key Lab Agroprod Proc Minist Agr Beijing 100193 Peoples R China 

出 版 物：《JOURNAL OF COLLOID AND INTERFACE SCIENCE》 (胶体与界面科学杂志)

年 卷 期：2014年第431卷

页      面：71-76页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：National Program on Key Basic Research Project of China (973 Program) [2013CB127804] National Natural Science Foundation of China [21205098, 21273173] Fundamental Research Funds for the Central Universities [XDJK2012C049] Visiting Scholarship of State Key Laboratory of Power Transmission Equipment & System Security and New Technology (Chongqing University) [2007DA10512712409] Institute for Clean Energy & Advanced Materials (Southwest University) Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Start-up Grant rom Southwest University Chongqing Engineering Research Center for Rapid Diagnosis of Dread Disease and Chongqing development and reform commission 

主　　题：Surface plasmon resonance imaging Immunoassay Mycotoxin Gold nanoparticles Signal amplification 

摘      要：Detection of multiple toxic mycotoxins is of importance in food quality control. Surface plasmon resonance imaging (SPRi) is an advanced tool for simultaneously multiple detections with accuracy;however, it suffers from limited sensitivity due to the instrumental constraint and small sizes of mycotoxins with only one epitope for an insensitive competitive immunoassay. In this work a gold nanoparticle (AuNP)-enhanced SPRi chip *** to sensitively detect multiple mycotoxins using a competitive immunoassay format. The sensing surface is constructed by uniformly attaching dense mycotoxin antigens on poly[oligo(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) brush modified SPRi gold chip. After competitive binding in a sample solution containing respective monoclonal antibodies, secondary antibody-conjugated AuNPs are employed to bind with the captured monoclonal antibodies for further amplification of the SPRi signal. Highly specific and sensitive simultaneous detection is achieved for three typical mycotoxins including Aflatoxin B1 (AFB1), Ochratoxin A (OTA) and Zearalenone (ZEN) with low detection limits of 8, 30 and 15 pg mL(-1) and dynamic ranges covering three orders of magnitude. (C) 2014 Elsevier Inc. All rights reserved.