Reduction of pulmonary mast cells in areas of acute inflammation in calves with <i>Mannheimia</i> (<i>Pasteurella</i>) <i>haemolytica</i> pneumonia

作     者：Ramírez-Romero, R Brogden, KA Gallup, JM Dixon, RAF Ackermann, MR 

作者机构：Iowa State Univ Coll Vet Med Dept Vet Pathol Ames IA 50011 USA USDA ARS Natl Anim Dis Ctr Ames IA 50010 USA Texas Biotechnol Corp Houston TX 77030 USA 

出 版 物：《JOURNAL OF COMPARATIVE PATHOLOGY》 (比较病理学杂志)

年 卷 期：2000年第123卷第1期

页      面：29-35页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 0906[农学-兽医学] 09[农学] 

基　　金：USDA/NRI, (970– 2654) Consejo Nacional de Ciencia y Tecnología, CONACYT Universidad Autónoma de Nuevo León, UANL 

主　　题：急性病 联苯化合物/治疗应用 细胞计数/兽医学 荧光抗体技术 直接/兽医学 肺/免疫学 肺/病理学 溶血性曼氏杆菌 甘露糖苷类/治疗应用 肥大细胞/免疫学 肥大细胞/病理学 巴斯德菌病 肺/药物疗法 巴斯德菌病 肺/免疫学 巴斯德菌病 肺/病理学 动物 牛 男(雄)性 

摘      要：Mast cells in the left cranial pulmonary lobe of colostrum-deprived neonatal calves were quantified 2 and 6 h after intrabronchial inoculation with Mannheimia (Pasteurella) haemolytica Al. The mast cells were detected ii) immunohistochemically with a mouse anti-human mast cell tryptase monoclonal antibody, and (2) by metachromatic staining with low pH toluidine blue. A greater number of mast cells was demonstrated by the second method than by the first. At 6 h after inoculation, but not at 2 h, the number of mast cells was significantly reduced at the site of the main lesions. Treatment of calves with a sialyl Lewis mimetic (TBC 1269) did not appreciably affect the results at 6 h. (C) 2000 Harcourt Publishers Ltd.