BmToll9, an Arthropod conservative Toll, is likely involved in the local gut immune response in the silkworm, <i>Bombyx mori</i>

作     者：Wu, Shan Zhang, Xiaofeng Chen, Xiaomei Cao, Pingsheng Beerntsen, Brenda T. Ling, Erjun 

作者机构：Chinese Acad Sci Grad Sch Res Ctr Insect Sci Inst Plant Physiol & EcolShanghai Inst Biol Sci Shanghai 200032 Peoples R China Zhejiang Entry Exit Inspect & Quarantine Bur Hangzhou 310012 Zhejiang Peoples R China Univ Missouri Dept Vet Pathobiol Coll Vet Meterinary Med Columbia MO 65211 USA 

出 版 物：《DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY》 (发育免疫学与比较免疫学)

年 卷 期：2010年第34卷第2期

页      面：93-96页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 0908[农学-水产] 0906[农学-兽医学] 10[医学] 

基　　金：Shanghai Post-Doctoral Research Project [07R214152] National Hi-Tech Research and Development Program of China [2006AA10A119] National Basic Research Program of China [2007CB513107] 

主　　题：BmToll9 RT-qPCR Expression Bombyx mori Sequence conservation Arthropods 

摘      要：The Toll family of transmembrane proteins mediates signaling during the innate immune response in most animals. Toll9 is widespread in insects and has a unique signature, QHR, in its Toll/interleukin-1 receptor (TIR) domain. The introns in the TIR region are highly conserved among insects, suggesting the antiquity of Toll9 genes. Toll9 of Bombyx mori (BmToll9) was analysed by quantitative real-time RT-PCR. BmToll9 is constitutively expressed in egg, larval and adult stages prior to microbial challenge. BmToll9 is strongly expressed in the different parts of the gut, but weakly expressed in haemocytes, trachea, fat body, malpighian tubule and epidermis, and scarcely expressed in the silk glands. The injection of sterilized 0.85% NaCl solution inhibited BmToll9 expression in most tissues especially during the early responses. Staphylococcus aureus had no or limited effect on the expression of BmToll9 in the silkworm gut and fat body. But in epidermis, trachea, malpighian tubules and haemocytes, the expression of BmToll9 was significantly increased after S. aureus challenge. Infection of Escherichia coli significantly increased the BmToll9 expression in different parts of the gut as well as in epidermis, malpighian tubule and haemocytes. At 48 h after feeding of the fungus, Beauveria bassiana, BmToll9 expression was significantly increased. tissues responses to the injected and ingested bacteria showed that BmToll9 is probably involved in the local gut immune response in the silkworm. (C) 2009 Elsevier Ltd. All rights reserved.