Analysis of Phytochelatins and Other Thiol-Containing Compounds by RP-HPLC with Monobromobimane Precolumn Derivatization

作     者：Sun Qin Ye Zhihong Wang Xiaorong Wong Minghong 

作者机构：College of Environmental Science and EngineeringHohai UniversityNanjing 210098PRC State Key Laboratory of Pollution Control and Resources ReuseSchool of the EnvironmentNanjing UniversityNanjing 210093PRC Croucher Institute for Environmental Science School of Life SciencesZhongshan UniversityGuangzhou 510275PRC State Key Laboratory of Pollution Control and Resources ReuseSchool of the EnvironmentNanjing UniversityNanjing 210093PRC Croucher Institute for Environmental Sciences and Department of BiologyHong Kong Baptist UniversityHong Kong SARPRC 

出 版 物：《中国高等学校学术文摘·化学》 (FRONTIERS OF CHEMISTRY IN CHINA)

年 卷 期：2006年第1卷第1期

页      面：54-58页

学科分类：07[理学] 0703[理学-化学] 

基　　金：the Ecogeochemical Investigation Foundation of Jiangsu Province,China(200312300008,20031230009-03) the Natural Science Foundation of Jiangsu Province,China(BK2004091) 

主　　题：phytochelatins thiol-containing compounds RP-HPLC precolumn derivatization monobromobimane Cd hyperaccumulator Sedum alfredii 

摘      要：In this article,a method for quantitative determination ofphytochelatins(PCn being the classic example)and other thiol-containing compounds in mixed standard solution and plant tissues is *** were converted to fluorescent derivatives by precolumn derivatization with *** results showed that PCn and other thiol-containing compounds in standard mixed solutions were rapidly separated within 15 min by using a ACN 0.1% trifluoroacetic acid binary gradient *** was representatively selected to test the precision of this *** calibration curve was linear in the range of 1.25-160 ng μl-1(regression coefficient r2=0.9999).It was confirmed that this method was rapid,simple,highly sensitive,stable,and had the property of simultaneous determination of PCn and other thiol-containing *** method was applied to determine PCn and other thiol-containing compounds in a Cd hyperaccumulator Sedum alfredii in response to *** was found that no PCn was detected in any tissue at any Cd treatment,suggesting that Cd hyperaccumulation and detoxification in this plant is not based on PC synthesis.