Untargeted LC-MS based <SUP>13</SUP>C labelling provides a full mass balance of deoxynivalenol and its degradation products formed during baking of crackers, biscuits and bread

作     者：Stadler, David Lambertini, Francesca Bueschl, Christoph Wiesenberger, Gerlinde Hametner, Christian Schwartz-Zimmermann, Heidi Hellinger, Roland Sulyok, Michael Lemmens, Marc Schuhmacher, Rainer Suman, Michele Berthiller, Franz Krska, Rudolf 

作者机构：Univ Nat Resources & Life Sci Vienna BOKU Ctr Analyt Chem Dept Agrobiotechnol IFA Tulln Konrad Lorenz Str 20 A-3430 Tulln Austria Queens Univ Belfast Sch Biol Sci Inst Global Food Secur Univ Rd Belfast BT7 1NN Antrim North Ireland Barilla GRF Lli SpA Adv Res Lab Via Mantova 166 I-43122 Parma Italy Univ Nat Resources & Life Sci Vienna BOKU Dept Appl Genet & Cell Biol Konrad Lorenz Str 24 A-3430 Tulln Austria Vienna Univ Technol Inst Appl Synthet Chem Getreidemarkt 9-163-OC A-1060 Vienna Austria Univ Nat Resources & Life Sci Vienna BOKU Inst Biotechnol Plant Prod Dept Agrobiotechnol IFA Tulln Konrad Lorenz Str 24 A-3430 Tulln Austria 

出 版 物：《FOOD CHEMISTRY》 (食品化学)

年 卷 期：2019年第279卷

页      面：303-311页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 0703[理学-化学] 

基　　金：European Union's Horizon 2020 research and innovation programme Austrian Science Fund (FWF) [SFB F3702] Government of Lower Austria (project NoBiTUM) H2020 Societal Challenges Programme Funding Source: H2020 Societal Challenges Programme 

主　　题：Mycotoxins Trichothecenes Thermal degradation Decontamination Metabolomics Mass spectrometry Food processing 

摘      要：Deoxynivalenol (DON) is considered to be one of the most important contaminants in cereals and food cornmodifies produced thereof. So far it is not clear i) to which extent DON is degraded during baking and ii) if a degradation results in reduced toxicity. We have elucidated the fate of DON during baking of crackers, biscuits and bread, which were produced from fortified dough and processed under pilot plant conditions. Untargeted stable isotope assisted liquid chromatography (LC) high resolution mass spectrometry was used to determine all extractable degradation products. Targeted LC - tandem mass spectrometry based quantification revealed that DON was partially degraded to isoDON (1.3-3.9%), norDON B (0.2-0.9%) and norDON C (0.3-1.2%). A DON degradation of 6% (crackers), 5% (biscuits) and 2% (bread), respectively, was observed. In vitro translation experiments indicate that isoDON is less toxic than DON.