Restriction accessibility in isolated nuclei reveals light-induced chromatin reorganization at the PEPC promoter in maize

作     者：Kalamajka, R Hahnen, S Cavalar, M Töpsch, S Weier, D Peterhänsel, C 

作者机构：Univ Aachen Inst Biol 1 D-52056 Aachen Germany 

出 版 物：《PLANT MOLECULAR BIOLOGY》 (植物分子生物学)

年 卷 期：2003年第52卷第3期

页      面：669-678页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：Deutsche Forschungsgemeinschaft  DFG 

主　　题：C-4 photosynthesis chromatin nuclei PEPC promoter real-time PCR 

摘      要：Expression of genes necessary to perform C-4 photosynthesis in maize is activated by light. It is not known how this activation is regulated on the chromatin level in vivo. We analysed alterations in the chromatin structure of the promoter of the C-4-specific isoform of phosphoenolpyruvate carboxylase (PEPC) after illumination of seedlings. A protocol was established that facilitates the preparation of nuclei from maize leaves with intact chromatin structure and resistance to DNA degradation during prolonged incubation at high temperatures. The presence of non-spliced transcripts from the C-4-PEPC gene in the nuclei was demonstrated by RT-PCR. The chromatin was partially digested with restriction endonucleases. Quantitative PCR analyses revealed a clear increase in the accessibility of the promoter chromatin to restriction dependent on illumination of the seedlings. The data indicate chromatin reorganization at the C-4-PEPC promoter during activation.