Adaptive optics for enhanced signal in CARS microscopy

作     者：Wright, A. J. Poland, S. P. Girkin, J. M. Freudiger, C. W. Evans, C. L. Xie, X. S. 

作者机构：Univ Strathclyde Inst Photon SUPA Glasgow G4 0NW Lanark Scotland Harvard Univ Dept Chem & Biol Chem Cambridge MA 02138 USA 

出 版 物：《OPTICS EXPRESS》 (Opt. Express)

年 卷 期：2007年第15卷第26期

页      面：18209-18219页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：EPSRC [EP/F020155/1] Funding Source: UKRI 

主　　题：.penetration depth aberration signal intensity optimization algorithm random search Coherent Anti-Stokes Raman CARS microscopy CARS signal deformable membrane mirror muscle tissue deep tissue 

摘      要：We report the use of adaptive optics with coherent anti-Stokes Raman scattering (CARS) microscopy for label-free deep tissue imaging based on molecular vibrational spectroscopy. The setup employs a deformable membrane mirror and a random search optimization algorithm to improve signal intensity and image quality at large sample depths. We demonstrate the ability to correct for both system and sample-induced aberrations in test samples as well as in muscle tissue in order to enhance the CARS signal. The combined system and sample- induced aberration correction increased the signal by an average factor of similar to 3x for the test samples at a depth of 700 mu m and similar to 6x for muscle tissue at a depth of 260 mu m. The enhanced signal and higher penetration depth offered by adaptive optics will augment CARS microscopy as an in vivo and in situ biomedical imaging modality. (c) 2007 Optical Society of America.