Immobilizing laccase on kaolinite and its application in treatment of malachite green effluent with the coexistence of Cd (П)

作     者：Xiaofeng Wen Chunyan Du Jia Wan Guangming Zeng Danlian Huang Lingshi Yin Rui Deng Shiyang Tan Jinfan Zhang 

作者机构：College of Environmental Science and Engineering Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China. Electronic address: wenxf0105@***. School of Hydraulic Engineering Changsha University of Science &Technology Changsha 410114 PR China Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province Changsha 410114 PR China. Electronic address: cydu@***. College of Environmental Science and Engineering Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China. Electronic address: wanjia@***. College of Environmental Science and Engineering Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China. Electronic address: zgming@***. College of Environmental Science and Engineering Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China. Electronic address: huangdanlian@***. Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province Changsha 410114 PR China. Electronic address: yin@***. College of Environmental Science and Engineering Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University) Ministry of Education Changsha 410082 PR China. Electronic address: dengrui703@***. Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province Changsha 410114 PR China. Electronic address: tan_shiyang0518@***. Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province Changsha 410114 PR China. Electronic address: yiyangzjf@***. 

出 版 物：《Chemosphere》 

年 卷 期：2019年第217卷

页      面：843-850页

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

主　　题：Cd (П) Coexistence Kaolinite Laccase immobilization Malachite green Removal 

摘      要：Malachite green effluent with the Coexistence of Cd (П) was efficiently decolorized by kaolinite-laccase (Kaolin-Lac). Laccase from Trametes versicolor was immobilized onto the kaolinite through physical adsorption contact. The optimal conditions were 180 min of immobilization time and 0.8 mg/mL of enzyme solution. Kaolin-Lac could obtain a loading efficiency of 88.22%, a loading capacity of 12.25 mg/g, and the highest activity of 839.01 U/g. Moreover, the process of immobilization increased its pH stability and operational stability. Kaolin-Lac retained above 50% of the original activity and nearly 80% decolorization for MG after 5 cycles. In the presence of 3, 5-Dimethoxy-4-hydroxybenzaldehyde (SA), Kaolin-Lac could degrade over 98% of malachite green. The coexistence of Cd (П) was beneficial to the decolorization of malachite green by Kaolin-Lac. The structural and morphological features of kaolinite, Kaolin-Lac and Kaolin-Lac after degradation were determined by scanning electron microscopy-energy spectrum analysis (SEM-EDS) and Fourier transform infrared spectroscopy (FTIR). Cadmium appeared on the Kaolin-Lac after degradation. After immobilization and degradation, the surface groups on kaolinite were changed. Kaolin-Lac showed its more potential continuous employment than free laccase in practical malachite green dyes effluent mixed with Cd (П).