<i>In vitro</i> and <i>in vivo</i> expression of endothelial von Willebrand factor and leukocyte accumulation after fractionated irradiation

作     者：van Kleef, E Verheij, M Poele, HT Oussoren, Y Dewit, L Stewart, F 

作者机构：Netherlands Canc Inst Div Expt Therapy NL-1066 CX Amsterdam Netherlands Netherlands Canc Inst Div Radiotherapy NL-1066 CX Amsterdam Netherlands 

出 版 物：《RADIATION RESEARCH》 (辐射研究)

年 卷 期：2000年第154卷第4期

页      面：375-381页

核心收录：

学科分类：0710[理学-生物学] 070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 1009[医学-特种医学] 0702[理学-物理学] 

主　　题：细胞黏附 细胞 培养的/代谢 细胞 培养的/辐射效应 趋化性 白细胞/辐射效应 内皮 血管/代谢 内皮 血管/辐射效应 肾皮质/代谢 肾皮质/病理学 肾皮质/辐射效应 肾功能试验 肾小球/代谢 肾小球/病理学 肾小球/辐射效应 肾小管/代谢 肾小管/病理学 肾小管/辐射效应 白细胞/病理学 小鼠 近交C3H 肾单位/代谢 肾单位/病理学 肾单位/辐射效应 辐射损伤 实验性/代谢 辐射损伤 实验性/病理学 辐射耐受性 脐静脉 vonWillebrand因子/生物合成 vonWillebrand因子/遗传学 动物 女(雌)性 人类 小鼠 

摘      要：Previous investigations have demonstrated an increased release of von Willebrand factor (VWF;also known as VWF) in endothelial cells after high single-dose irradiation in vitro. We have also found increased levels of Vwf protein in mouse glomeruli after a high single dose of renal irradiation in vivo. In addition, increased numbers of leukocytes were observed in the renal cortex after irradiation in vivo. The aim of the present study was to investigate and quantify these biological processes after clinically relevant fractionated irradiation and to relate them to changes in renal function. A significantly greater increase in release of VWF was observed in cultured human umbilical vein endothelial cells (HUVECs) after fractionated irradiation (20 x 1.0 Gy) than after a single dose of 20 Gy (147% compared to 115% of control, respectively, P 0.0005). In contrast with the in vitro observations, glomerular Vwf staining was lower after fractionated irradiation in vivo (20 x 2.0 Gy or 10 x 1.6 Gy +/- re-irradiation) than after a single dose of 16 Gy. The number of leukocytes accumulating in the renal cortex was also lower after fractionated in vivo irradiation than after a single radiation dose. The onset of these events preceded renal functional and histopathological changes by approximately 10 weeks. These data indicate that radiation-induced changes in endothelial. VWF expression after in vivo irradiation may be distinct from the in vitro observations. Increased VWF expression may reflect pivotal processes in the pathogenesis of late radiation nephropathy and provide a clue to appropriate timing of pharmacological intervention. (C) 2000 by Radiation Research Society.